PA5-30003

antibody from Invitrogen Antibodies

Targeting: CUX1 CASP, CDP, CDP/Cut, CDP/Cux, CDP1, Clox, CUT, CUTL1, CUX, Cux/CDP, GOLIM6

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30003

Provider

Invitrogen Antibodies

Product name

CUX1/Protein CASP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: Raji, K562. Predicted reactivity: Human (99%), Mouse (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Overlapping Role of SCYL1 and SCYL3 in Maintaining Motor Neuron Viability.

Kuliyev E, Gingras S, Guy CS, Howell S, Vogel P, Pelletier S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Mar 7;38(10):2615-2630

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CUX1/Protein CASP Polyclonal Antibody detects CUTL1 protein at Golgi apparatus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTL1 protein stained by CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CUX1/Protein CASP Polyclonal Antibody detects CUTL1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTL1 stained by CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CUX1/Protein CASP Polyclonal Antibody detects CUTL1 protein at Golgi apparatus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTL1 protein stained by CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CUX1/Protein CASP Polyclonal Antibody detects CUTL1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUTL1 stained by CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CUX1/Protein CASP Polyclonal Antibody immunoprecipitates CUTL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of CUTL1 protein by 2 µg of CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) 7.5% SDS-PAGE The immunoprecipitated CUTL1 protein was detected by CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) diluted at 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CUX1/Protein CASP Polyclonal Antibody immunoprecipitates CUTL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of CUTL1 protein by 2 µg of CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) 7.5% SDS-PAGE The immunoprecipitated CUTL1 protein was detected by CUX1/Protein CASP Polyclonal Antibody (Product # PA5-30003) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. COPI and CASP interactions contribute to localization of SCYL3 to the Golgi apparatus. A , Generation of CASP-deficient MEFs using CRISPR-Cas9 technology. Structure of the Cux1 gene encoding CDP and CASP. Exons common to CDP and CASP are illustrated in black (only exons 12-14 illustrated). Exons encoding CDP are illustrated in green, and those encoding CASP are illustrated in blue. To generate CASP-deficient cells, 2 sgRNAs (black arrowheads) were designed to delete exons 15-17. Splicing of exon 14 into exon 18 causes a frame shift and premature stop codon. Red arrow, Cux1-F51 primer; green arrow, Cux-R32 primer. B , PCR genotyping of the WT and 2 independently derived CASP-deficient MEF lines (Cl.7 and Cl.8). Bands of 2496 and ~495 bp correspond to WT and CASP- alleles, respectively. A smaller band (*) from internal priming sites was also obtained by using these primers. NTC, No template control. Sanger sequencing confirmed proper recombination of the allele in CASP-deficient MEFs. C , Western blot analysis of CASP and beta-actin (as loading control) in WT and CASP-deficient MEFs. *Indicates nonspecific bands. D , Immunofluorescence staining of CASP and GM130 in WT and CASP-deficient MEFs. Exponentially growing WT and CASP-deficient MEFs were fixed and stained for CASP and GM130 and imaged by confocal microscopy. E , SCYL3 localization in WT and CASP-deficient MEFs. Exponentially growing WT and CASP-deficient MEFs were fixed and s