Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-22646 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD34 Monoclonal Antibody (MEC14.7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody detects a neuraminidase sensitive epitope on endothelium in vivo, particullary on small vessels and neoformed capillaries and develpoing vascular structures in embryonal structures. Furthermore, the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.
- Reactivity
- Human, Mouse, Rabbit
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- MEC14.7
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects.
Molecular changes in solitary fibrous tumor progression.
Enhanced endothelial motility and multicellular sprouting is mediated by the scaffold protein TKS4.
Inflammatory neovascularization during graft-versus-host disease is regulated by αv integrin and miR-100.
The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.
Myeloproliferative defects following targeting of the Drf1 gene encoding the mammalian diaphanous related formin mDia1.
Li Q, Zhao F, Li Z, Duan X, Cheng J, Zhang J, Fu X, Zhang J, Shao Z, Guo Q, Hu X, Ao Y
Frontiers in cell and developmental biology 2020;8:694
Frontiers in cell and developmental biology 2020;8:694
Molecular changes in solitary fibrous tumor progression.
Park HK, Yu DB, Sung M, Oh E, Kim M, Song JY, Lee MS, Jung K, Noh KW, An S, Song K, Nam DH, Kim YJ, Choi YL
Journal of molecular medicine (Berlin, Germany) 2019 Oct;97(10):1413-1425
Journal of molecular medicine (Berlin, Germany) 2019 Oct;97(10):1413-1425
Enhanced endothelial motility and multicellular sprouting is mediated by the scaffold protein TKS4.
Mehes E, Barath M, Gulyas M, Bugyik E, Geiszt M, Szoor A, Lanyi A, Czirok A
Scientific reports 2019 Oct 7;9(1):14363
Scientific reports 2019 Oct 7;9(1):14363
Inflammatory neovascularization during graft-versus-host disease is regulated by αv integrin and miR-100.
Leonhardt F, Grundmann S, Behe M, Bluhm F, Dumont RA, Braun F, Fani M, Riesner K, Prinz G, Hechinger AK, Gerlach UV, Dierbach H, Penack O, Schmitt-Gräff A, Finke J, Weber WA, Zeiser R
Blood 2013 Apr 25;121(17):3307-18
Blood 2013 Apr 25;121(17):3307-18
The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.
Paz H, Lynch MR, Bogue CW, Gasson JC
Blood 2010 Aug 26;116(8):1254-62
Blood 2010 Aug 26;116(8):1254-62
Myeloproliferative defects following targeting of the Drf1 gene encoding the mammalian diaphanous related formin mDia1.
Peng J, Kitchen SM, West RA, Sigler R, Eisenmann KM, Alberts AS
Cancer research 2007 Aug 15;67(16):7565-71
Cancer research 2007 Aug 15;67(16):7565-71
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry staining of mouse H5V cells using CD34 antibody (Product # MA1-22646) and purified rat IgG2a isotype control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry staining of mouse H5V cells using CD34 antibody (Product # MA1-22646) and purified rat IgG2a isotype control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 Multi-lineage differentiation of cultured ADSCs migrated from the ECM/SVF-gel. (A) Cultured primary ADSCs from the ECM/SVF-gel exhibited a typical spindle shape morphology. (B) Chondrogenic, (C) osteogenic, and (D) adipogenic differentiation of ADSCs was induced and determined by Alcian blue staining for glycosaminoglycans, Alizarin red staining for matrix mineralization, and Oil Red O staining for intracellular lipid droplets, respectively. Scale bars: (A-C) = 200 mum; (D) = 100 mum. (E) Flow cytometry analysis of surface marker on ADSCs from the ECM/SVF-gel.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Characterization of the molecular and histopathological features of the index SFT case. a Diagram of the primary and metastatic sites of the index case. b A schematic pre-mRNAs of the fusion variant 2a from the RNA-sequencing experiment. Bottom sequences in black are the reads that map onto the chimeric exon-exon splicing junction. c The fusion variant 2a was confirmed by RT-PCR and Sanger sequencing. d Comparison of CD34, Ki-67, and STAT6 immunohistochemical staining and counterpart H&E staining in the primary and metastatic tissues
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 Chorion vessel analysis. Representative fluorescent microscopic images of chorions from ( a ) WT or ( b ) Tks4-KO embryos where endothelial cells were visualized by CD34 immunofluorescent labeling (green), scale bar: 100 mu m. Quantitative analysis of vessel parameters in 3 WT and 3 Tks4-KO individuals: ( c ) vessel density, ( d ) average width of vessels, ( e ) total length of all vessels in a 865 mu m x 650 mu m microscopic field. Error bars correspond to SEM and asterisks (*) indicate statistically significant differences with Student's t-test, \documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{upgreek}\setlength{\oddsidemargin}{-69pt}\begin{document}$$p < 0.05$$\end{document} p < 0.05 .
