Explore
Validate
LearnNB100-78614
antibody from Novus Biologicals
Targeting: PDIA3
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-78614 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-78614, RRID:AB_1084624
- Product name
- Rabbit Polyclonal ERp57/PDIA3 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:10000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - A. 20 ug rat liver lysate/extract 7.5 % SDS-PAGE ERp57 antibody [C3], C-term dilution: 1:10000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - H1299 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Rat middle brain. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Mouse intestine. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Paraffin-embedded mouse lymph node. ERp57 antibody [C3], C-term dilution: 1:500.
