NB110-41576

antibody from Novus Biologicals

Targeting: ABHD5 CGI-58, NCIE2

Provider product page for NB110-41576

Western blot

Antibody data

Antibody Data
Antigen structure
References [25]
Comments [0]
Validations
Western blot [5]

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Product number: NB110-41576 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB110-41576, RRID:AB_828505
Product name: Rabbit Polyclonal Abhd5 Antibody
Antibody type: Polyclonal
Description: IgG purified.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

ABHD5 protein structure - NB110-41576 shown in red.

Submitted references Assessment of the Main Compounds of the Lipolytic System in Treadmill Running Rats: Different Response Patterns between the Right and Left Ventricle.

Mikłosz A, Łukaszuk B, Baranowski M, Chabowski A, Górski J
International journal of molecular sciences 2019 May 24;20(10)

Distinct lipid droplet characteristics and distribution unmask the apparent contradiction of the athlete's paradox.

Daemen S, Gemmink A, Brouwers B, Meex RCR, Huntjens PR, Schaart G, Moonen-Kornips E, Jörgensen J, Hoeks J, Schrauwen P, Hesselink MKC
Molecular metabolism 2018 Nov;17:71-81

OPA1-anchored PKA phosphorylates perilipin 1 on S522 and S497 in adipocytes differentiated from human adipose stem cells.

Rogne M, Chu DT, Küntziger TM, Mylonakou MN, Collas P, Tasken K
Molecular biology of the cell 2018 Jun 15;29(12):1487-1501

Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle.

Perreault L, Newsom SA, Strauss A, Kerege A, Kahn DE, Harrison KA, Snell-Bergeon JK, Nemkov T, D'Alessandro A, Jackman MR, MacLean PS, Bergman BC
JCI insight 2018 Feb 8;3(3)

Prior Endurance Training Enhances Beta-Adrenergic Signaling in Epidydimal Adipose from Mice Fed a High-Fat Diet.

Snook LA, Trottier SK, Worndl EA, Bombardier E, Tupling AR, MacPherson REK
Obesity (Silver Spring, Md.) 2017 Oct;25(10):1699-1706

Lipid droplet dynamics and insulin sensitivity upon a 5-day high-fat diet in Caucasians and South Asians.

Gemmink A, Bakker LE, Guigas B, Kornips E, Schaart G, Meinders AE, Jazet IM, Hesselink MK
Scientific reports 2017 Feb 14;7:42393

Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles.

Turnbull PC, Longo AB, Ramos SV, Roy BD, Ward WE, Peters SJ
American journal of physiology. Regulatory, integrative and comparative physiology 2016 Jan 15;310(2):R125-33

Lipid mixtures containing a very high proportion of saturated fatty acids only modestly impair insulin signaling in cultured muscle cells.

Newsom SA, Everett AC, Park S, Van Pelt DW, Hinko A, Horowitz JF
PloS one 2015;10(3):e0120871

Characterization of lipolytic inhibitor G(0)/G(1) switch gene-2 protein (G0S2) expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase (ATGL) and comparitive gene identification-58 (CGI-58).

Turnbull PC, Ramos SV, MacPherson RE, Roy BD, Peters SJ
PloS one 2015;10(3):e0120136

A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood.

MacPherson RE, Castelli LM, Miotto PM, Frendo-Cumbo S, Milburn A, Roy BD, LeBlanc PJ, Ward WE, Peters SJ
Lipids 2015 Feb;50(2):205-17

Transgenic CGI-58 expression in macrophages alleviates the atherosclerotic lesion development in ApoE knockout mice.

Xie P, Zeng X, Xiao J, Sun B, Yang D
Biochimica et biophysica acta 2014 Dec;1841(12):1683-90

Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation.

Macpherson RE, Vandenboom R, Roy BD, Peters SJ
Physiological reports 2013 Sep;1(4):e00084

Skeletal muscle triacylglycerol hydrolysis does not influence metabolic complications of obesity.

Sitnick MT, Basantani MK, Cai L, Schoiswohl G, Yazbeck CF, Distefano G, Ritov V, DeLany JP, Schreiber R, Stolz DB, Gardner NP, Kienesberger PC, Pulinilkunnil T, Zechner R, Goodpaster BH, Coen P, Kershaw EE
Diabetes 2013 Oct;62(10):3350-61

Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle.

Alsted TJ, Ploug T, Prats C, Serup AK, Høeg L, Schjerling P, Holm C, Zimmermann R, Fledelius C, Galbo H, Kiens B
The Journal of physiology 2013 Oct 15;591(20):5141-55

IL-6 and epinephrine have divergent fiber type effects on intramuscular lipolysis.

Macdonald TL, Wan Z, Frendo-Cumbo S, Dyck DJ, Wright DC
Journal of applied physiology (Bethesda, Md. : 1985) 2013 Nov;115(10):1457-63

PPARγ coactivator-1α contributes to exercise-induced regulation of intramuscular lipid droplet programming in mice and humans.

Koves TR, Sparks LM, Kovalik JP, Mosedale M, Arumugam R, DeBalsi KL, Everingham K, Thorne L, Phielix E, Meex RC, Kien CL, Hesselink MK, Schrauwen P, Muoio DM
Journal of lipid research 2013 Feb;54(2):522-34

Inflammation produces catecholamine resistance in obesity via activation of PDE3B by the protein kinases IKKε and TBK1.

Mowers J, Uhm M, Reilly SM, Simon J, Leto D, Chiang SH, Chang L, Saltiel AR
eLife 2013 Dec 24;2:e01119

Overexpression of PLIN5 in skeletal muscle promotes oxidative gene expression and intramyocellular lipid content without compromising insulin sensitivity.

Bosma M, Sparks LM, Hooiveld GJ, Jorgensen JA, Houten SM, Schrauwen P, Kersten S, Hesselink MK
Biochimica et biophysica acta 2013 Apr;1831(4):844-52

Skeletal muscle PLIN proteins, ATGL and CGI-58, interactions at rest and following stimulated contraction.

MacPherson RE, Ramos SV, Vandenboom R, Roy BD, Peters SJ
American journal of physiology. Regulatory, integrative and comparative physiology 2013 Apr 15;304(8):R644-50

Valsartan improves adipose tissue function in humans with impaired glucose metabolism: a randomized placebo-controlled double-blind trial.

Goossens GH, Moors CC, van der Zijl NJ, Venteclef N, Alili R, Jocken JW, Essers Y, Cleutjens JP, Clément K, Diamant M, Blaak EE
PloS one 2012;7(6):e39930

Perilipin 2 improves insulin sensitivity in skeletal muscle despite elevated intramuscular lipid levels.

Bosma M, Hesselink MK, Sparks LM, Timmers S, Ferraz MJ, Mattijssen F, van Beurden D, Schaart G, de Baets MH, Verheyen FK, Kersten S, Schrauwen P
Diabetes 2012 Nov;61(11):2679-90

Alternative splicing and developmental and hormonal regulation of porcine comparative gene identification-58 (CGI-58) mRNA.

Li X, Suh Y, Kim E, Moeller SJ, Lee K
Journal of animal science 2012 Dec;90(12):4346-54

Paradoxical increase in TAG and DAG content parallel the insulin sensitizing effect of unilateral DGAT1 overexpression in rat skeletal muscle.

Timmers S, de Vogel-van den Bosch J, Hesselink MK, van Beurden D, Schaart G, Ferraz MJ, Losen M, Martinez-Martinez P, De Baets MH, Aerts JM, Schrauwen P
PloS one 2011 Jan 14;6(1):e14503

Skeletal muscle lipase content and activity in obesity and type 2 diabetes.

Jocken JW, Moro C, Goossens GH, Hansen D, Mairal A, Hesselink MK, Langin D, van Loon LJ, Blaak EE
The Journal of clinical endocrinology and metabolism 2010 Dec;95(12):5449-53

Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training.

Alsted TJ, Nybo L, Schweiger M, Fledelius C, Jacobsen P, Zimmermann R, Zechner R, Kiens B
American journal of physiology. Endocrinology and metabolism 2009 Mar;296(3):E445-53

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: Abhd5 Antibody [NB110-41576] - Image shows a specific band for Abhd5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Abhd5 Antibody [NB110-41576] - Abhd5 overexpression lysates (NBL1-07199).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Abhd5 Antibody [NB110-41576] - MCF7 cell lysates.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Abhd5 Antibody [NB110-41576] - CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Abhd5 Antibody [NB110-41576] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of ATGL. In all figure panels data are expressed relative to a no fatty acid control condition (0mM). In panel B, #P