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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP31810PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP31810PU-N, RRID:AB_11148329
- Product name
- anti Nestin
- Antibody type
- Polyclonal
- Antigen
- Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence.Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
- Reactivity
- Mouse, Rat
- Host
- Chicken/Avian
- Vial size
- 0.3 mg
- Concentration
- 0.3 mg/ml (based on Bradford assay readings using BSA as a standard).
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- A tissue section through an e13 Mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei.
