AM00036PU-N

antibody from Acris Antibodies GmbH

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AM00036PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00036PU-N, RRID:AB_980924

Product name

anti EGFR / ERBB1 pSer1071 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Phosphopeptide conjugated to KLH

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

1H9

Vial size

0.1 mg

References

Submitted references

Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells.

Nishi H, Maeda N, Izumi S, Higa-Nakamine S, Toku S, Kakinohana M, Sugahara K, Yamamoto H
European journal of pharmacology 2015 Feb 5;748:133-42

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

EGFR Transactivation: Serum starved HepG2 cells were treated for 15min as indicated . Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-1H9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: Control Lane 2: PMA Lane 3: Forskolin Lane 4: LPALane 5: Sorbit Lane 6; Anisomycin Lane 7: Ionomycin Lane 8: Taxol