AM00055PU-N

antibody from Acris Antibodies GmbH

Targeting: ERBB3 HER3, LCCS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM00055PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00055PU-N, RRID:AB_977221

Product name

anti ERBB3 / HER3 (Cytopl. Dom.) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Peptide conjugated to hemocyanin (aa 1250-1270).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5A12

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous erbB3: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB3-5A12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620