AM00077PU-N

antibody from Acris Antibodies GmbH

Targeting: INSR CD220

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM00077PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00077PU-N, RRID:AB_975467

Product name

anti CD220 / INSR (beta chain) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic peptide conjugated to KLH.Epitope: Kinase Activation Loop

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

9H4

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous InsR: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-9H4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D