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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- R1567P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1567P, RRID:AB_973810
- Product name
- anti Caspase-2
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.67 mg/ml (by UV absorbance at 280 nm)
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Figure 1. Immunoblotting. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kDa caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a HRP conjugated Gt-a-Rabbit IgG (R1454HRP) preceeded color development using Amersham's substrate system.
