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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Flow cytometry [3]
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Validation data
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- Product number
- BM4008R - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#BM4008R, RRID:AB_1004813
- Product name
- anti Macrophage F4/80 antigen
- Antibody type
- Monoclonal
- Antigen
- Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- CI:A3-1
- Vial size
- 100 Tests
Submitted references Claudin-3 and Clara cell 10 kDa protein as early signals of cigarette smoke-induced epithelial injury along alveolar ducts.
Cuzić S, Bosnar M, Kramarić MD, Ferencić Z, Marković D, Glojnarić I, Eraković Haber V
Toxicologic pathology 2012 Dec;40(8):1169-87
Toxicologic pathology 2012 Dec;40(8):1169-87
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4008F (5 µg/ml) on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (BM4008R), red and Rat anti Mouse CD45, green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence image of lymph node stained for macrophages (BM4008R), red and B cells CD79b, green; nuclei are stained with DAPI, blue
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometry: Staining of Mouse J774 cells with RPE conjugated Rat Anti Mouse F4/80 Antigen (BM4008R).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen (BM4008R)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4008F on thioglycollate elicited mouse peritoneal macrophages. Purple: BM4008F; Green: Isotype control SM19FS; Percentages reflect % positive after subtraction of negative control, using M1 marker.
