Explore
Validate
LearnAP12803PU-N
antibody from Acris Antibodies GmbH
Targeting: RPS6KB1
p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP12803PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12803PU-N, RRID:AB_1770200
- Product name
- anti RPS6KB1 / STK14A pSer424
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser424 of human RPS6KB1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. (LEFT) Western blot analysis of Phospho-RPS6KB1-pSer424 Antibody (Cat#AP12803PU-N) and RPS6KB1 Antibody (Ser424) (Cat#AP12744PU-N) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (Cat#AP12803PU-N); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (Cat#AP12744PU-N). (RIGHT) Dot blot analysis of anti-RPS6KB1-pSer424 Phospho-specific Pab (Cat#AP12803PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
