AP09320PU-N

antibody from Acris Antibodies GmbH

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09320PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09320PU-N, RRID:AB_2035448

Product name

anti EGFR / ERBB1 pTyr1197

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 1189-1199 of human EGFR protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.55 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-EGFR pY1197 antibody shows detection of a band at ~170 kDa corresponding to human EGFR (arrowhead). Staining is not seen in unstimulated A431 cells (lane 1), but is seen when A431 cells are stimulated with EGF (50 ng/ml for 15 min) (lane 2). Approximately 30 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Affinity purified anti-EGFR pY1197 antibody was used at 5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multicancer slides. This image shows faintly to moderately positive staining of placental trophoblasts at 40X. Tissue was formalinfixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.