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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP19047PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP19047PU-N, RRID:AB_10669007
- Product name
- anti CHK1 pSer345
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide contain a sequence corresponding to a region within amino acids 292 and 379 of CHK1 phopho-Ser345
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP19047PU-N CHK1 antibody (pSer345) staining of HTC116 P53 null whole cell lysate 10% SDS PAGE (20 µg) at 1/1000 dilution.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of whole cell lysate (30 µg) using AP19047PU-N CHK1 antibody (pSer345) at 1/500 dilution. 10% SDS PAGEA: HTC116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AP19047PU-N CHK1 antibody (pSer345) (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1/2000.
