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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- AM00283AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00283AF-N, RRID:AB_10756859
- Product name
- anti Bax
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide (KLH conjugated) corresponding to amino acids 3â16 near the N-terminus of Mouse Bax.
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5B7
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Bax expression in HL60 cells (Lane 1), Raji cells (Lane 2), MCF7 cells (Lane 3), A431 cells (Lane 4), WR19L cells (Lane 5), C2C12 cells (Lane 6), NIH/3T3 cells (Lane 7), Rat-1 cells (Lane 8), BaF/3 cells (Lane 9) and PC12 cells (Lane 10) using Bax antibody AM00283AF-N
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of Bax and Bcl-xL from WR19L cells with AM00283AF-N (Lane 2 and Lane 6), Mouse IgG1 (Lane 3) or Mouse IgG2a (Lane 5). After immunoprecipition with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM00283AF-N (Lane 1, 2 and 3) or BcL-xL monoclonal antibody AM20181AF-N (Lane 4, 5 and 6). WR19L crude lysate was resolved in Lane 1 and 4.
