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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NB500-210 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-210, RRID:AB_10002924
- Product name
- Mouse Monoclonal Caspase-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease.
2-Oxonanonoidal Antibiotic Actinolactomycin Inhibits Cancer Progression by Suppressing HIF-1α.
Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-).
Differential effects of angiotensin receptor blockers on pancreatic islet remodelling and glucose homeostasis in diet-induced obese mice.
The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer.
Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer.
Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients.
Hypoxia-inducible factors enhance glutamate signaling in cancer cells.
Sann-Joong-Kuey-Jian-Tang inhibits hepatocellular carcinoma Hep-G2 cell proliferation by increasing TNF-α, Caspase-8, Caspase- 3 and Bax but by decreasing TCTP and Mcl-1 expression in vitro.
TNF receptor-associated factor 6 regulates proliferation, apoptosis, and invasion of glioma cells.
Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells.
Regulation of TGF-β1 expression by androgen deprivation therapy of prostate cancer.
Induction of apoptosis in human breast adenocarcinoma cells MCF-7 by monapurpyridine A, a new azaphilone derivative from Monascus purpureus NTU 568.
Apogossypol derivative BI-97C1 (Sabutoclax) targeting Mcl-1 sensitizes prostate cancer cells to mda-7/IL-24-mediated toxicity.
Anti-tumor and anti-inflammatory properties of ankaflavin and monaphilone A from monascus purpureus NTU 568.
Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma.
Tissue biomarkers for prognosis in cutaneous melanoma: a systematic review and meta-analysis.
Tumor cell-selective regulation of NOXA by c-MYC in response to proteasome inhibition.
Caspase-9 and APAF-1 form an active holoenzyme.
Caspase-9 and APAF-1 form an active holoenzyme.
Rutledge EA, Parvez RK, Short KM, Smyth IM, McMahon AP
Developmental biology 2019 Oct 15;454(2):156-169
Developmental biology 2019 Oct 15;454(2):156-169
2-Oxonanonoidal Antibiotic Actinolactomycin Inhibits Cancer Progression by Suppressing HIF-1α.
Cheng J, Hu L, Yang Z, Suo C, Wang YJ, Gao P, Cui C, Sun L
Cells 2019 May 10;8(5)
Cells 2019 May 10;8(5)
Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-).
Martins FF, Aguila MB, Mandarim-de-Lacerda CA
Acta histochemica 2017 Jun;119(5):508-515
Acta histochemica 2017 Jun;119(5):508-515
Differential effects of angiotensin receptor blockers on pancreatic islet remodelling and glucose homeostasis in diet-induced obese mice.
Graus-Nunes F, Marinho TS, Barbosa-da-Silva S, Aguila MB, Mandarim-de-Lacerda CA, Souza-Mello V
Molecular and cellular endocrinology 2017 Jan 5;439:54-64
Molecular and cellular endocrinology 2017 Jan 5;439:54-64
The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer.
Hsuan SW, Chyau CC, Hung HY, Chen JH, Chou FP
European journal of nutrition 2016 Mar;55(2):491-503
European journal of nutrition 2016 Mar;55(2):491-503
Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer.
Kim H, Lee KH, Park IA, Chung YR, Im SA, Noh DY, Han W, Moon HG, Jung YY, Ryu HS
Virchows Archiv : an international journal of pathology 2015 Nov;467(5):563-70
Virchows Archiv : an international journal of pathology 2015 Nov;467(5):563-70
Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients.
Shibayama Y, Kondo T, Ohya H, Fujisawa S, Teshima T, Iseki K
Oncology reports 2015 May;33(5):2176-82
Oncology reports 2015 May;33(5):2176-82
Hypoxia-inducible factors enhance glutamate signaling in cancer cells.
Hu H, Takano N, Xiang L, Gilkes DM, Luo W, Semenza GL
Oncotarget 2014 Oct 15;5(19):8853-68
Oncotarget 2014 Oct 15;5(19):8853-68
Sann-Joong-Kuey-Jian-Tang inhibits hepatocellular carcinoma Hep-G2 cell proliferation by increasing TNF-α, Caspase-8, Caspase- 3 and Bax but by decreasing TCTP and Mcl-1 expression in vitro.
Chen YL, Yan MY, Chien SY, Kuo SJ, Chen DR, Cheng CY, Su CC
Molecular medicine reports 2013 May;7(5):1487-93
Molecular medicine reports 2013 May;7(5):1487-93
TNF receptor-associated factor 6 regulates proliferation, apoptosis, and invasion of glioma cells.
Peng Z, Shuangzhu Y, Yongjie J, Xinjun Z, Ying L
Molecular and cellular biochemistry 2013 May;377(1-2):87-96
Molecular and cellular biochemistry 2013 May;377(1-2):87-96
Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells.
Chien SY, Kuo SJ, Chen DR, Su CC
International journal of molecular medicine 2013 Jul;32(1):85-92
International journal of molecular medicine 2013 Jul;32(1):85-92
Regulation of TGF-β1 expression by androgen deprivation therapy of prostate cancer.
Fuzio P, Ditonno P, Rutigliano M, Battaglia M, Bettocchi C, Loverre A, Grandaliano G, Perlino E
Cancer letters 2012 May 28;318(2):135-44
Cancer letters 2012 May 28;318(2):135-44
Induction of apoptosis in human breast adenocarcinoma cells MCF-7 by monapurpyridine A, a new azaphilone derivative from Monascus purpureus NTU 568.
Hsu LC, Hsu YW, Liang YH, Liaw CC, Kuo YH, Pan TM
Molecules (Basel, Switzerland) 2012 Jan 11;17(1):664-73
Molecules (Basel, Switzerland) 2012 Jan 11;17(1):664-73
Apogossypol derivative BI-97C1 (Sabutoclax) targeting Mcl-1 sensitizes prostate cancer cells to mda-7/IL-24-mediated toxicity.
Dash R, Azab B, Quinn BA, Shen X, Wang XY, Das SK, Rahmani M, Wei J, Hedvat M, Dent P, Dmitriev IP, Curiel DT, Grant S, Wu B, Stebbins JL, Pellecchia M, Reed JC, Sarkar D, Fisher PB
Proceedings of the National Academy of Sciences of the United States of America 2011 May 24;108(21):8785-90
Proceedings of the National Academy of Sciences of the United States of America 2011 May 24;108(21):8785-90
Anti-tumor and anti-inflammatory properties of ankaflavin and monaphilone A from monascus purpureus NTU 568.
Hsu LC, Hsu YW, Liang YH, Kuo YH, Pan TM
Journal of agricultural and food chemistry 2011 Feb 23;59(4):1124-30
Journal of agricultural and food chemistry 2011 Feb 23;59(4):1124-30
Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma.
Kulkarni AA, Kingsbury SR, Tudzarova S, Hong HK, Loddo M, Rashid M, Rodriguez-Acebes S, Prevost AT, Ledermann JA, Stoeber K, Williams GH
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 1;15(7):2417-25
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 1;15(7):2417-25
Tissue biomarkers for prognosis in cutaneous melanoma: a systematic review and meta-analysis.
Gould Rothberg BE, Bracken MB, Rimm DL
Journal of the National Cancer Institute 2009 Apr 1;101(7):452-74
Journal of the National Cancer Institute 2009 Apr 1;101(7):452-74
Tumor cell-selective regulation of NOXA by c-MYC in response to proteasome inhibition.
Nikiforov MA, Riblett M, Tang WH, Gratchouck V, Zhuang D, Fernandez Y, Verhaegen M, Varambally S, Chinnaiyan AM, Jakubowiak AJ, Soengas MS
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 4;104(49):19488-93
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 4;104(49):19488-93
Caspase-9 and APAF-1 form an active holoenzyme.
Rodriguez J, Lazebnik Y
Genes & development 1999 Dec 15;13(24):3179-84
Genes & development 1999 Dec 15;13(24):3179-84
Caspase-9 and APAF-1 form an active holoenzyme.
Rodriguez J, Lazebnik Y
Genes & development 1999 Dec 15;13(24):3179-84
Genes & development 1999 Dec 15;13(24):3179-84
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Lane view shows a specific band for Caspase 3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Detection of Caspase (19 and 35 kDa) from HEK293 cell extract using Caspase-3 Antibody (CPP32 4-1-18) (NB500-210). Lanes 1 and 2 contain inactive and active Caspase, respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder 1:300 dilution of mouse monoclonal Caspase-3 Antibody (CPP32 4-1-18) (NB500-210, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Analysis of a FFPE human spleen section using 1:200 dilution of Caspase-3 Antibody (CPP32 4-1-18). The staining was developed using HRP conjugated anti-mouse secondary antibody and DAB reagent. This Caspase 3 antibody generated a specific staining in the cytoplasm of various spleenocytes.
