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LearnAP21619SU-S
antibody from Acris Antibodies GmbH
Targeting: BCL2L1
Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP21619SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21619SU-S, RRID:AB_10758597
- Product name
- anti Bcl-2-like 1
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to amino acids 46-66 (ETPSAINGNPSWHLADSPAVN) of human Bcl-XL was
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Bcl-X in lysates from isolated rat liver mitochondria using AP21619SU-S at 1/2000. The antibody detected Bcl-XL at ~30 kDa.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Analysis of Bcl-X in human breast carcinoma using AP21619SU-S at 1/2000. A. Formalin-fixed, paraffin-embedded tissue section from invasive ductal carcinoma. An organellar/mitochondrial pattern of Bcl-X staining is observed in the cytosol of tumor cells. B. Western blot analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is is detected. Lane 8. Recombinant Bcl-XL (positive control).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-X expression using AP21619SU-S. Visualized with DAB (A) or VIP (B) chromagens. Hematoxylin-eosin counterstain. The staining results show that the basal (germinal) layer at the bottom of the images were negative for Bcl-X expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-X expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-X expression.
