AP21656SU-S

antibody from Acris Antibodies GmbH

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP21656SU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP21656SU-S, RRID:AB_10758596

Product name

anti Caspase-3 (Pro and Active)

Antibody type

Polyclonal

Antigen

Recombinant full-length human Caspase-3 protein (pro-form)

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Vial size

50 µl

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of Caspase-3. Lysates from Jurkat cells (lane 1), normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas (lanes 3-5)were normalized for total protein content (50 µg/lane) and western blotted with anti-Caspase-3 (AP21656SU-S). The ~32 kDa pro-Caspase-3 protein was detected in all samples. Active/cleaved Caspase-3 was identified in Jurkat (10 kDa small subunit, lane 1) and two ductal carcinomas (14-21 kDa large subunit).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human reactive lymph node using AP21656SU-S at 1/2000. Staining is seen in the apoptosis-prone germinal center B lymphocytes of follicles. In constrast little or no staining is seen in the surrounding long-live mantle zone lymphocytes.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ using AP21656SU-S at 1/2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.