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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP21673PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21673PU-N, RRID:AB_10757766
- Product name
- anti Cyclin D1
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acid 250 to 300 of human CCND1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of CCND1 in 1) mouse C2C12 and 2) mouse NIH 3T3 cell lysate using AP21673PU-N at 5 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCND1 in formalin-fixed, paraffin-embedded human stomach tissue using an isotype control (top left) and AP21673PU-N (bottom left, right) at 5 µg/ml.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody (Cat.-No.: AP21673PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
