GTX25612

antibody from GeneTex

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX25612

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25612, RRID:AB_380595

Product name

MEK1 (phospho Thr292) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of NIH3T3 cells untreated (lane 1) or treated with 50 ng/mL PDGF for 15 minutes (lanes 2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 4% BSA-TBST buffer for one hour at room temperature, then incubated with the MEK1 [pT292] antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1, 2), a generic phosphothreonine-containing peptide (3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab¡¦)2 anti-rabbit IgG HRP conjugate and signals were detected. The data show that only the phosphopeptide corresponding to MEK1 [pT292] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of MEK1 [pT292] phosphorylation by the addition of PDGF to this cell system. In addition, this antibody did not recognize a recombinant MEK1 T292A mutant protein, further demonstrating its specificity for MEK1 [pT292] (data not shown).