NB110-85534

antibody from Novus Biologicals

Targeting: FAP DPPIV

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

NB110-85534

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-85534, RRID:AB_1201235

Product name

Rabbit Polyclonal Fibroblast Activation Protein alpha/FAP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% identity with the immunizing sequence.

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

VCAM-1 secreted from cancer-associated fibroblasts enhances the growth and invasion of lung cancer cells through AKT and MAPK signaling.

Zhou Z, Zhou Q, Wu X, Xu S, Hu X, Tao X, Li B, Peng J, Li D, Shen L, Cao Y, Yang L
Cancer letters 2020 Mar 31;473:62-73

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Antitumor immunity targeting fibroblast activation protein-α in a mouse Lewis lung carcinoma model.

Xie J, Yuan S, Peng L, Li H, Niu L, Xu H, Guo X, Yang M, Duan F
Oncology letters 2020 Jul;20(1):868-876

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Analysis of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at 90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.