Explore
Validate
LearnMAB665
antibody from R&D Systems
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB665 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGFR2 (IIIb) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Reacts with multiple isoforms of human FGF R2 although it shows a marked preference for human FGF R2 (IIIb) isoforms in some assays as described below. In direct ELISAs, displays a three-fold preference for FGF R2 (IIIb) isoforms over (IIIc) isoforms. Shows approximately 50% cross-reactivity with recombinant mouse (rm) FGF R2 isoforms and no cross-reactivity with any isoforms of recombinant human (rh) FGF R1 or rhFGF R3. In Western blots, shows approximately 25% cross-reactivity with rhFGF R2 (IIIc) isoforms, 100% cross-reactivity with all isoforms of rmFGF R2 and no cross-reactivity with any isoforms of rhFGF RI, rhFGF R3, or rhFGF R4. Neutralizes the bioactivity of rhFGF R2 (IIIb) isoforms.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 98707
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Translocation of fibroblast growth factor-10 and its receptor into nuclei of human urothelial cells.
Kosman J, Carmean N, Leaf EM, Dyamenahalli K, Bassuk JA
Journal of cellular biochemistry 2007 Oct 15;102(3):769-85
Journal of cellular biochemistry 2007 Oct 15;102(3):769-85
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (Catalog # 663-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 (IIIb) Monoclonal Antibody (Catalog # MAB665). The ND50 is typically 2-8 µg/mL in the presence of heparin (10 µg/mL).
