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LearnNB100-293
antibody from Novus Biologicals
Targeting: RPS6KB1
p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- NB100-293 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-293, RRID:AB_10002810
- Product name
- Rabbit Polyclonal p70 S6 Kinase/S6K Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references REDD1 (regulated in development and DNA damage response 1) expression in skeletal muscle as a surrogate biomarker of the efficiency of glucocorticoid receptor blockade.
Downregulation of Akt/mammalian target of rapamycin pathway in skeletal muscle is associated with increased REDD1 expression in response to chronic hypoxia.
Arginine activates intestinal p70(S6k) and protein synthesis in piglet rotavirus enteritis.
Progressive loss of epidermal growth factor receptor in a subpopulation of breast cancers: implications in target-directed therapeutics.
Intestinal ribosomal p70(S6K) signaling is increased in piglet rotavirus enteritis.
Role of mTOR signaling in intestinal cell migration.
Kumari R, Willing LB, Jefferson LS, Simpson IA, Kimball SR
Biochemical and biophysical research communications 2011 Sep 9;412(4):644-7
Biochemical and biophysical research communications 2011 Sep 9;412(4):644-7
Downregulation of Akt/mammalian target of rapamycin pathway in skeletal muscle is associated with increased REDD1 expression in response to chronic hypoxia.
Favier FB, Costes F, Defour A, Bonnefoy R, Lefai E, Baugé S, Peinnequin A, Benoit H, Freyssenet D
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Jun;298(6):R1659-66
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Jun;298(6):R1659-66
Arginine activates intestinal p70(S6k) and protein synthesis in piglet rotavirus enteritis.
Corl BA, Odle J, Niu X, Moeser AJ, Gatlin LA, Phillips OT, Blikslager AT, Rhoads JM
The Journal of nutrition 2008 Jan;138(1):24-9
The Journal of nutrition 2008 Jan;138(1):24-9
Progressive loss of epidermal growth factor receptor in a subpopulation of breast cancers: implications in target-directed therapeutics.
Choong LY, Lim S, Loh MC, Man X, Chen Y, Toy W, Pan M, Chen CS, Poonepalli A, Hande MP, Tan PH, Salto-Tellez M, Wong CY, Shah N, Druker BJ, Lim YP
Molecular cancer therapeutics 2007 Nov;6(11):2828-42
Molecular cancer therapeutics 2007 Nov;6(11):2828-42
Intestinal ribosomal p70(S6K) signaling is increased in piglet rotavirus enteritis.
Rhoads JM, Corl BA, Harrell R, Niu X, Gatlin L, Phillips O, Blikslager A, Moeser A, Wu G, Odle J
American journal of physiology. Gastrointestinal and liver physiology 2007 Mar;292(3):G913-22
American journal of physiology. Gastrointestinal and liver physiology 2007 Mar;292(3):G913-22
Role of mTOR signaling in intestinal cell migration.
Rhoads JM, Niu X, Odle J, Graves LM
American journal of physiology. Gastrointestinal and liver physiology 2006 Sep;291(3):G510-7
American journal of physiology. Gastrointestinal and liver physiology 2006 Sep;291(3):G510-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Rat p70S6 Kinase by Western Blot. Sample: Lysate from rat L6 myoblast that were serum starved for 1.5 h (lane 1) or serum-deprived for 1 h and then treated with 20 ng/ml IGF-1 for 30 minutes (lane 2). Antibody: Affinity purified rabbit anti-p70S6 kinase (NB100-293) used at 0.1 mcg/ml. Detection: Chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
