GTX630201

antibody from GeneTex

Targeting: NES FLJ21841

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX630201

Provider

GeneTex

Product name

Nestin antibody [GT623]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

References

Submitted references

Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.

Mansel C, Fross S, Rose J, Dema E, Mann A, Hart H, Klawinski P, Vohra BPS
Neurotoxicology and teratology 2019 Mar - Apr;72:58-70

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Biochemical re-programming of human dermal stem cells to neurons by increasing mitochondrial membrane potential.

Liu H, He Z, April SL, Trefny MP, Rougier JS, Salemi S, Olariu R, Widmer HR, Simon HU
Cell death and differentiation 2018 Aug 28;

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Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model.

Xie MQ, Chen ZC, Zhang P, Huang HJ, Wang TT, Ding YQ, Qi SS, Zhang C, Chen SX, Zhou P, Shao CC, Liao M, Sun CY
Neuroscience 2017 Jun 3;352:64-78

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Improved Proliferative Capacity of NP-Like Cells Derived from Human Mesenchymal Stromal Cells and Neuronal Transdifferentiation by Small Molecules.

Aguilera-Castrejon A, Pasantes-Morales H, Montesinos JJ, Cortés-Medina LV, Castro-Manrreza ME, Mayani H, Ramos-Mandujano G
Neurochemical research 2017 Feb;42(2):415-427

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Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus.

Pringproa K, Rungsiwiwut R, Tantilertcharoen R, Praphet R, Pruksananonda K, Baumgärtner W, Thanawongnuwech R
PloS one 2015;10(8):e0135850

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin Antibody (GTX630201) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Nestin antibody [GT623] detects Nestin protein at intermediate filament by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde for 10 min.Green: Nestin protein stained by Nestin antibody [GT623] (GTX630201) diluted at 1:300.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nestin protein stained by Nestin antibody [GT623] (GTX630201) diluted at 1:250.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.