M802B

antibody from Invitrogen Antibodies

Targeting: CXCL8 3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1

ELISA (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

M802B

Provider

Invitrogen Antibodies

Product name

IL-8 (CXCL8) Monoclonal Antibody (I8-S2), Biotin

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

M802B targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.

Conjugate

Biotin

Antibody clone number

I8-S2

Concentration

0.5 mg/mL

References

Submitted references

Chronic effects of two rutile TiO(2) nanomaterials in human intestinal and hepatic cell lines.

Jalili P, Krause BC, Lanceleur R, Burel A, Jungnickel H, Lampen A, Laux P, Luch A, Fessard V, Hogeveen K
Particle and fibre toxicology 2022 May 17;19(1):37

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Inflammatory cytokines tumor necrosis factor-α, interleukin-8 and sleep monitoring in patients with obstructive sleep apnea syndrome.

Ming H, Tian A, Liu B, Hu Y, Liu C, Chen R, Cheng L
Experimental and therapeutic medicine 2019 Mar;17(3):1766-1770

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Combined effects of okadaic acid and pectenotoxin-2, 13-desmethylspirolide C or yessotoxin in human intestinal Caco-2 cells.

Alarcan J, Barbé S, Kopp B, Hessel-Pras S, Braeuning A, Lampen A, Le Hégarat L, Fessard V
Chemosphere 2019 Aug;228:139-148

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Resolvin D2 decreases TLR4 expression to mediate resolution in human monocytes.

Croasdell A, Sime PJ, Phipps RP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Sep;30(9):3181-93

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Microparticles engineered to highly express peroxisome proliferator-activated receptor-γ decreased inflammatory mediator production and increased adhesion of recipient monocytes.

Sahler J, Woeller CF, Phipps RP
PloS one 2014;9(11):e113189

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The human salivary proteome is radiation responsive.

Moore HD, Ivey RG, Voytovich UJ, Lin C, Stirewalt DL, Pogosova-Agadjanyan EL, Paulovich AG
Radiation research 2014 May;181(5):521-30

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Attenuation of inflammatory mediator production by the NF-κB member RelB is mediated by microRNA-146a in lung fibroblasts.

McMillan DH, Woeller CF, Thatcher TH, Spinelli SL, Maggirwar SB, Sime PJ, Phipps RP
American journal of physiology. Lung cellular and molecular physiology 2013 Jun 1;304(11):L774-81

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Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.

Salem S, Harris T, Mok JS, Li MY, Keenan CR, Schuliga MJ, Stewart AG
British journal of pharmacology 2012 Aug;166(7):2036-48

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Optical protein sensor for detecting cancer markers in saliva.

Tan W, Sabet L, Li Y, Yu T, Klokkevold PR, Wong DT, Ho CM
Biosensors & bioelectronics 2008 Oct 15;24(2):266-71

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IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Saffar AS, Alphonse MP, Shan L, Hayglass KT, Simons FE, Gounni AS
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2535-41

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IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Saffar AS, Alphonse MP, Shan L, Hayglass KT, Simons FE, Gounni AS
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2535-41

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Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, Legay F
Journal of immunological methods 2006 Oct 20;316(1-2):1-7

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Regulation of myeloid cell function through the CD200 receptor.

Jenmalm MC, Cherwinski H, Bowman EP, Phillips JH, Sedgwick JD
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan 1;176(1):191-9

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Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms.

Beigi RD, Kertesy SB, Aquilina G, Dubyak GR
British journal of pharmacology 2003 Oct;140(3):507-19

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Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, Fietta A, Vitulo P, Pozzi E, Viganò M
Transplantation proceedings 2003 Jun;35(4):1523-6

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Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, Fietta A, Vitulo P, Pozzi E, Viganò M
Transplantation proceedings 2003 Jun;35(4):1523-6

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Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease.

Urbanowska T, Mangialaio S, Hartmann C, Legay F
Cell biology and toxicology 2003 Jun;19(3):189-202

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Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease.

Urbanowska T, Mangialaio S, Hartmann C, Legay F
Cell biology and toxicology 2003 Jun;19(3):189-202

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Regulation of interleukin 8 expression in human malignant melanoma cells.

Singh RK, Varney ML
Cancer research 1998 Apr 1;58(7):1532-7

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA analysis of human IL-8 was performed using a Human IL-8 Colorimetric ELISA kit (Product # EH2IL8) by loading 50 µL per well of Human IL-8 Recombinant Protein (Product # SIL8) in dodecuplicate at 1000, 400, 160, 64, 25.6, and 0 pg/mL across a 3 µg/mL mouse anti-Human IL-8 (Product # M801) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, and then incubated with 50 µL per well of a biotinylated mouse anti-human IL-8 monoclonal antibody (Product # M802B) in duplicate at 0.25 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at a 1:4,000 dilution for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

Conjugate

Biotin

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Microparticle composition influences inflammatory cytokine production from activated monocytes. THP-1 cells ( A ) or CD14 sorted blood monocytes ( B ) were treated with control microparticles (grey bars) or PPARgamma-overexpressing microparticles (black bars) or no microparticles (white bars) for 4 hours before activation with LPS or PAM3CSK4 for 24 hours. Supernatants were collected and pro-inflammatory cytokines IL-8 (top), MCP-1 (bottom) were measured by ELISA. Dotted line represents baseline cytokine production from unactivated cells with no microparticle exposure. Mean values with standard errors represent one of at least 3 experiments. Two-way ANOVA with Tukey's multiple comparison post test was performed to determine statistical significance. * indicates (p

Conjugate

Biotin