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LearnIC208F
antibody from R&D Systems
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC208F - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-8/CXCL8 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCL8/IL-8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL-8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 beta .
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P10145- Isotype
- IgG
- Antibody clone number
- 6217
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Expression of the T cell receptor αβ on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
PloS one 2011 Jan 11;6(1):e15997
PloS one 2011 Jan 11;6(1):e15997
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
Méndez-Cruz AR, Paez A, Jiménez-Flores R, Reyes-Reali J, Varela E, Cerbulo-Vazquez A, Rodriguez E, López-Marure R, Masso FA, Flores-Romo L, Montaño LF
Immunology letters 2007 Aug 15;111(2):116-23
Immunology letters 2007 Aug 15;111(2):116-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCL8/IL-8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC208F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P), as shown in panel A. Inhibition of IC208F staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
