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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN1842262 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Rat, Bovine, Porcine
- Host
- Rat
- Vial size
- 100 μL
Submitted references Purification of two distinct proteins of approximate Mr 80,000 from human epithelial cells and identification as proper substrates for protein kinase C.
Hirai M, Shimizu N
The Biochemical journal 1990 Sep 15;270(3):583-9
The Biochemical journal 1990 Sep 15;270(3):583-9
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot of whole rat cortex, cerebellum, brain stem and spinal cord homogenate stained with RPCA- MARCKS, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~80 kDa corresponds to MARCKS.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- High Magnification view of mixed neuron/glial cultures stained with our MARCKS antibody (red) and CPCA-MAP2, antibody to MAP2 made in chicken. Blue is DNA stain. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are stained with the MAP2 antibody.
