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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB14167 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14167, RRID:AB_10553661
- Product name
- MARCKS polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant MARCKS.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MARCKS deficiency in mice leads to abnormal brain development and perinatal death.
The MARCKS family of cellular protein kinase C substrates.
MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin.
Stumpo DJ, Bock CB, Tuttle JS, Blackshear PJ
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 14;92(4):944-8
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 14;92(4):944-8
The MARCKS family of cellular protein kinase C substrates.
Blackshear PJ
The Journal of biological chemistry 1993 Jan 25;268(3):1501-4
The Journal of biological chemistry 1993 Jan 25;268(3):1501-4
MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin.
Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC, Aderem A
Nature 1992 Apr 16;356(6370):618-22
Nature 1992 Apr 16;356(6370):618-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot of whole rat cortex (Lane 1), cerebellum (Lane 2), brain stem (Lane 3) and spinal cord (Lane 4) homogenate stained with MARCKS polyclonal antibody (Cat # PAB14167), at dilution of 1 : 10,000.A prominent band running with an apparent SDS-PAGE molecular weight of ~ 80 KDa corresponds to MARCKS.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- View of mixed neuron/glial cultures stained with MARCKS polyclonal antibody (Cat # PAB14167) (red) and Chicken MAP2 polyclonal antibody.Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
- Validation comment
- Immunofluorescence
