GTX128959

antibody from GeneTex

Targeting: H4C1 H4FA, HIST1H4A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX128959

Provider

GeneTex

Product name

Histone H4K20me1 (monomethyl Lys20) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein by Western blot analysis.A. 30 μg HeLa whole cell lysate/extractB. 30 μg HeLa nuclear lysate/extract15 % SDS-PAGEHistone H4 (mono methyl Lys20) antibody (GTX128959) dilution: 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody (GTX128959) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dotblot analysis of anti-Histone H4 (mono methyl Lys20) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (mono methyl Lys20) antibody (GTX128959) 1:3000 dilution.A: Peptide samples of H4K20 unB: Peptide samples of H4K20me1C: Peptide samples of H4K20me2D: Peptide samples of H4K20me3

Validation comment

Dot