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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- GTX128960 - Provider product page
- Provider
- GeneTex
- Product name
- Histone H4K20me3 (trimethyl Lys20) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein by Western blot analysis.A. 30 μg HeLa whole cell lysate/extractB. 30 μg HeLa nuclear lysate/extract15 % SDS-PAGEHistone H4 (tri methyl Lys20) antibody (GTX128960) dilution: 1:2000
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (GTX128960) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
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- GeneTex (provider)
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- Experimental details
- Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Dotblot analysis of anti-Histone H4 (tri methyl Lys20) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (tri methyl Lys20) antibody (GTX128960) 1:5000 dilution.A: Peptide samples of H4K20 unB: Peptide samples of H4K20me1C: Peptide samples of H4K20me2D: Peptide samples of H4K20me3
- Validation comment
- Dot
