NBP2-42863

antibody from Novus Biologicals

Targeting: H4-16 HIST4H4, MGC24116

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-42863

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Histone H4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Xenopus

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18 hrs) B. Control with 2.5 ug of pre-immune rabbit IgG C. Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 ug of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.

Other assay

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac.