Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- NB21-2000 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB21-2000, RRID:AB_11019163
- Product name
- Rabbit Polyclonal Histone H4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 0.89 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures.
Exon array analysis reveals neuroblastoma tumors have distinct alternative splicing patterns according to stage and MYCN amplification status.
Prediction of clinical outcome using gene expression profiling and artificial neural networks for patients with neuroblastoma.
Clouaire T, Rocher V, Lashgari A, Arnould C, Aguirrebengoa M, Biernacka A, Skrzypczak M, Aymard F, Fongang B, Dojer N, Iacovoni JS, Rowicka M, Ginalski K, Côté J, Legube G
Molecular cell 2018 Oct 18;72(2):250-262.e6
Molecular cell 2018 Oct 18;72(2):250-262.e6
Exon array analysis reveals neuroblastoma tumors have distinct alternative splicing patterns according to stage and MYCN amplification status.
Guo X, Chen QR, Song YK, Wei JS, Khan J
BMC medical genomics 2011 Apr 18;4:35
BMC medical genomics 2011 Apr 18;4:35
Prediction of clinical outcome using gene expression profiling and artificial neural networks for patients with neuroblastoma.
Wei JS, Greer BT, Westermann F, Steinberg SM, Son CG, Chen QR, Whiteford CC, Bilke S, Krasnoselsky AL, Cenacchi N, Catchpoole D, Berthold F, Schwab M, Khan J
Cancer research 2004 Oct 1;64(19):6883-91
Cancer research 2004 Oct 1;64(19):6883-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in HeLa histone prep.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: Histone H4 [p Ser1] Antibody [NB21-2000] - 2 ug of NB21-2000 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dot Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in picomoles of peptide.