Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations [0]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 39167 - Provider product page
- Provider
- Active Motif
- Proper citation
- Active Motif Cat#39167, RRID:AB_2636968
- Product name
- Histone H4K16ac antibody (pAb)
- Antibody type
- Polyclonal
- Description
- This Histone H4 acetyl Lys16 antibody (H4K16ac) has been validated for use in chromatin IP (3 - 10 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each) and Western blotting (1:1,000 - 1:5,000 dilution). Specificity for acetyl-lysine 16 was confirmed by dot blot and peptide inhibition with non-modified histone H4 peptide or peptides acetylated at lysines 5, 8 or 12. Because this modification is conserved from yeast to human, a wide range of reactivity is expected. Overnight incubation with this antibody is recommended for Western blotting. Due to cytoplasmic staining, this product is not recommended for IF.The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H4 acetyl Lys16 antibody was validated for ChIP-Seq in the study (see References).
- Reactivity
- Human, Drosophila, Zebrafish
- Host
- Rabbit
- Vial size
- 200 µl
- Storage
- Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
No comments: Submit comment
No validations: Submit validation data