Antibody data
- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14528-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14528-1-AP, RRID:AB_2303727
- Product name
- TOM70 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated TOM70 antibody (Cat. #14528-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Differential responses to folic acid in an established keloid fibroblast cell line are mediated by JAK1/2 and STAT3.
Hypothermia promotes mitochondrial elongation In cardiac cells via inhibition of Drp1.
Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
miR-34a-5p regulates PINK1-mediated mitophagy via multiple modes.
Dexamethasone upregulates mitochondrial Tom20, Tom70, and MnSOD through SGK1 in the kidney cells.
A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.
Intermittent Use of a Short-Course Glucagon-like Peptide-1 Receptor Agonist Therapy Limits Adverse Cardiac Remodeling via Parkin-dependent Mitochondrial Turnover.
Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.
A dual druggable genome-wide siRNA and compound library screening approach identifies modulators of parkin recruitment to mitochondria.
Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.
Antiviral Effects of Menthol on Coxsackievirus B.
ALS/FTD-associated protein FUS induces mitochondrial dysfunction by preferentially sequestering respiratory chain complex mRNAs.
LonP1 regulates mitochondrial network remodeling through the PINK1/Parkin pathway during myoblast differentiation.
EAE-induced upregulation of mitochondrial MnSOD is associated with increases of mitochondrial SGK1 and Tom20 protein in the mouse kidney cortex.
In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.
The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.
SIRT3 Activation by Dihydromyricetin Suppresses Chondrocytes Degeneration via Maintaining Mitochondrial Homeostasis.
Efficient proximity labeling in living cells and organisms with TurboID.
Yap regulates mitochondrial structural remodeling during myoblast differentiation.
Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.
GRSF1 suppresses cell senescence.
Mitophagy and mitochondrial biogenesis in atrial tissue of patients undergoing heart surgery with cardiopulmonary bypass.
Coxsackievirus B Escapes the Infected Cell in Ejected Mitophagosomes.
Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts.
Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number.
Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.
COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.
Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability.
Directed evolution of APEX2 for electron microscopy and proximity labeling.
Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation.
The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.
A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.
The protease Omi regulates mitochondrial biogenesis through the GSK3β/PGC-1α pathway.
Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
McCann KJ, Yadav M, Alishahedani ME, Freeman AF, Myles IA
PloS one 2021;16(3):e0248011
PloS one 2021;16(3):e0248011
Hypothermia promotes mitochondrial elongation In cardiac cells via inhibition of Drp1.
Taylor D, Germano J, Song Y, Hadj-Moussa H, Marek-Iannucci S, Dhanji R, Sin J, Czer LSC, Storey KB, Gottlieb RA
Cryobiology 2021 Oct;102:42-55
Cryobiology 2021 Oct;102:42-55
Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J
eLife 2021 May 26;10
eLife 2021 May 26;10
miR-34a-5p regulates PINK1-mediated mitophagy via multiple modes.
Tai Y, Pu M, Yuan L, Guo H, Qiao J, Lu H, Wang G, Chen J, Qi X, Tao Z, Ren J
Life sciences 2021 Jul 1;276:119415
Life sciences 2021 Jul 1;276:119415
Dexamethasone upregulates mitochondrial Tom20, Tom70, and MnSOD through SGK1 in the kidney cells.
Hira S, Packialakshmi B, Tang E, Zhou X
Journal of physiology and biochemistry 2021 Feb;77(1):1-11
Journal of physiology and biochemistry 2021 Feb;77(1):1-11
A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.
Fang H, Ye X, Xie J, Li Y, Li H, Bao X, Yang Y, Lin Z, Jia M, Han Q, Zhu J, Li X, Zhao Q, Yang Y, Lyu J
Cell reports 2021 Apr 13;35(2):108963
Cell reports 2021 Apr 13;35(2):108963
Intermittent Use of a Short-Course Glucagon-like Peptide-1 Receptor Agonist Therapy Limits Adverse Cardiac Remodeling via Parkin-dependent Mitochondrial Turnover.
Germano JF, Huang C, Sin J, Song Y, Tucker KC, Taylor DJR, Saadaeijahromi H, Stotland A, Piplani H, Gottlieb RA, Mentzer RM Jr, Andres AM
Scientific reports 2020 May 19;10(1):8284
Scientific reports 2020 May 19;10(1):8284
Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.
Wei X, Du M, Xie J, Luo T, Zhou Y, Zhang K, Li J, Chen D, Xu P, Jia M, Zhou H, Fang H, Lyu J, Yang Y
Journal of human genetics 2020 Mar;65(3):231-240
Journal of human genetics 2020 Mar;65(3):231-240
A dual druggable genome-wide siRNA and compound library screening approach identifies modulators of parkin recruitment to mitochondria.
Scott HL, Buckner N, Fernandez-Albert F, Pedone E, Postiglione L, Shi G, Allen N, Wong LF, Magini L, Marucci L, O'Sullivan GA, Cole S, Powell J, Maycox P, Uney JB
The Journal of biological chemistry 2020 Mar 6;295(10):3285-3300
The Journal of biological chemistry 2020 Mar 6;295(10):3285-3300
Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.
Phu L, Rose CM, Tea JS, Wall CE, Verschueren E, Cheung TK, Kirkpatrick DS, Bingol B
Molecular cell 2020 Mar 5;77(5):1107-1123.e10
Molecular cell 2020 Mar 5;77(5):1107-1123.e10
Antiviral Effects of Menthol on Coxsackievirus B.
Taylor DJR, Hamid SM, Andres AM, Saadaeijahromi H, Piplani H, Germano JF, Song Y, Sawaged S, Feuer R, Pandol SJ, Sin J
Viruses 2020 Mar 28;12(4)
Viruses 2020 Mar 28;12(4)
ALS/FTD-associated protein FUS induces mitochondrial dysfunction by preferentially sequestering respiratory chain complex mRNAs.
Tsai YL, Coady TH, Lu L, Zheng D, Alland I, Tian B, Shneider NA, Manley JL
Genes & development 2020 Jun 1;34(11-12):785-805
Genes & development 2020 Jun 1;34(11-12):785-805
LonP1 regulates mitochondrial network remodeling through the PINK1/Parkin pathway during myoblast differentiation.
Huang S, Wang X, Yu J, Tian Y, Yang C, Chen Y, Chen H, Ge H
American journal of physiology. Cell physiology 2020 Dec 1;319(6):C1020-C1028
American journal of physiology. Cell physiology 2020 Dec 1;319(6):C1020-C1028
EAE-induced upregulation of mitochondrial MnSOD is associated with increases of mitochondrial SGK1 and Tom20 protein in the mouse kidney cortex.
Hira S, Packialakshmi B, Zhou X
The journal of physiological sciences : JPS 2019 Sep;69(5):723-732
The journal of physiological sciences : JPS 2019 Sep;69(5):723-732
In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.
Momcilovic M, Jones A, Bailey ST, Waldmann CM, Li R, Lee JT, Abdelhady G, Gomez A, Holloway T, Schmid E, Stout D, Fishbein MC, Stiles L, Dabir DV, Dubinett SM, Christofk H, Shirihai O, Koehler CM, Sadeghi S, Shackelford DB
Nature 2019 Nov;575(7782):380-384
Nature 2019 Nov;575(7782):380-384
The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.
Hoshino A, Wang WJ, Wada S, McDermott-Roe C, Evans CS, Gosis B, Morley MP, Rathi KS, Li J, Li K, Yang S, McManus MJ, Bowman C, Potluri P, Levin M, Damrauer S, Wallace DC, Holzbaur ELF, Arany Z
Nature 2019 Nov;575(7782):375-379
Nature 2019 Nov;575(7782):375-379
SIRT3 Activation by Dihydromyricetin Suppresses Chondrocytes Degeneration via Maintaining Mitochondrial Homeostasis.
Wang J, Wang K, Huang C, Lin D, Zhou Y, Wu Y, Tian N, Fan P, Pan X, Xu D, Hu J, Zhou Y, Wang X, Zhang X
International journal of biological sciences 2018;14(13):1873-1882
International journal of biological sciences 2018;14(13):1873-1882
Efficient proximity labeling in living cells and organisms with TurboID.
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY
Nature biotechnology 2018 Oct;36(9):880-887
Nature biotechnology 2018 Oct;36(9):880-887
Yap regulates mitochondrial structural remodeling during myoblast differentiation.
Huang S, Wang X, Wu X, Yu J, Li J, Huang X, Zhu C, Ge H
American journal of physiology. Cell physiology 2018 Oct 1;315(4):C474-C484
American journal of physiology. Cell physiology 2018 Oct 1;315(4):C474-C484
Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.
Zhou B, Zhang JY, Liu XS, Chen HZ, Ai YL, Cheng K, Sun RY, Zhou D, Han J, Wu Q
Cell research 2018 Dec;28(12):1171-1185
Cell research 2018 Dec;28(12):1171-1185
GRSF1 suppresses cell senescence.
Noh JH, Kim KM, Idda ML, Martindale JL, Yang X, Abdelmohsen K, Gorospe M
Aging 2018 Aug 7;10(8):1856-1866
Aging 2018 Aug 7;10(8):1856-1866
Mitophagy and mitochondrial biogenesis in atrial tissue of patients undergoing heart surgery with cardiopulmonary bypass.
Andres AM, Tucker KC, Thomas A, Taylor DJ, Sengstock D, Jahania SM, Dabir R, Pourpirali S, Brown JA, Westbrook DG, Ballinger SW, Mentzer RM Jr, Gottlieb RA
JCI insight 2017 Feb 23;2(4):e89303
JCI insight 2017 Feb 23;2(4):e89303
Coxsackievirus B Escapes the Infected Cell in Ejected Mitophagosomes.
Sin J, McIntyre L, Stotland A, Feuer R, Gottlieb RA
Journal of virology 2017 Dec 15;91(24)
Journal of virology 2017 Dec 15;91(24)
Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts.
Sin J, Andres AM, Taylor DJ, Weston T, Hiraumi Y, Stotland A, Kim BJ, Huang C, Doran KS, Gottlieb RA
Autophagy 2016;12(2):369-80
Autophagy 2016;12(2):369-80
Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number.
Spadafora D, Kozhukhar N, Alexeyev MF
PloS one 2016;11(3):e0152705
PloS one 2016;11(3):e0152705
Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.
Xu S, Cherok E, Das S, Li S, Roelofs BA, Ge SX, Polster BM, Boyman L, Lederer WJ, Wang C, Karbowski M
Molecular biology of the cell 2016 Jan 15;27(2):349-59
Molecular biology of the cell 2016 Jan 15;27(2):349-59
COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.
Martinez Lyons A, Ardissone A, Reyes A, Robinson AJ, Moroni I, Ghezzi D, Fernandez-Vizarra E, Zeviani M
Journal of medical genetics 2016 Dec;53(12):846-849
Journal of medical genetics 2016 Dec;53(12):846-849
Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability.
Kang Y, Baker MJ, Liem M, Louber J, McKenzie M, Atukorala I, Ang CS, Keerthikumar S, Mathivanan S, Stojanovski D
eLife 2016 Aug 24;5
eLife 2016 Aug 24;5
Directed evolution of APEX2 for electron microscopy and proximity labeling.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY
Nature methods 2015 Jan;12(1):51-4
Nature methods 2015 Jan;12(1):51-4
Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation.
Kazlauskaite A, Martínez-Torres RJ, Wilkie S, Kumar A, Peltier J, Gonzalez A, Johnson C, Zhang J, Hope AG, Peggie M, Trost M, van Aalten DM, Alessi DR, Prescott AR, Knebel A, Walden H, Muqit MM
EMBO reports 2015 Aug;16(8):939-54
EMBO reports 2015 Aug;16(8):939-54
The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.
Bingol B, Tea JS, Phu L, Reichelt M, Bakalarski CE, Song Q, Foreman O, Kirkpatrick DS, Sheng M
Nature 2014 Jun 19;510(7505):370-5
Nature 2014 Jun 19;510(7505):370-5
A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.
Fiesel FC, Moussaud-Lamodière EL, Ando M, Springer W
Journal of cell science 2014 Aug 15;127(Pt 16):3488-504
Journal of cell science 2014 Aug 15;127(Pt 16):3488-504
The protease Omi regulates mitochondrial biogenesis through the GSK3β/PGC-1α pathway.
Xu R, Hu Q, Ma Q, Liu C, Wang G
Cell death & disease 2014 Aug 14;5:e1373
Cell death & disease 2014 Aug 14;5:e1373
Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW
Nature 2013 Apr 18;496(7445):372-6
Nature 2013 Apr 18;496(7445):372-6
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse liver tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP(TOM70 antibody) at dilution of 1:1500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using TOMM70A antibody 14528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-TOM70 (IP:14528-1-AP, 3ug; Detection:14528-1-AP 1:500) with mouse brain tissue lysate 6000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human heart using 14528-1-AP(TOM70 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human heart using 14528-1-AP(TOM70 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue