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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- ABIN1882250 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-H2A Histone Family, Member X (H2AFX) (C-Term), (AA 115-143) antibody
- Antibody type
- Monoclonal
- Antigen
- This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.
- Description
- This antibody is purified through a protein G column, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Epitope
- C-Term,AA 115-143
- Isotype
- IgG
- Antibody clone number
- 938CT5-1-1
- Vial size
- 400 μL
- Concentration
- 0.46 mg/mL
Submitted references DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner.
Park EJ, Chan DW, Park JH, Oettinger MA, Kwon J
Nucleic acids research 2003 Dec 1;31(23):6819-27
Nucleic acids research 2003 Dec 1;31(23):6819-27
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- H2AFX Antibody (C-term) (ABIN1882250) western blot analysis in 293,CEM,HepG2,Jurkat,Hela cell line lysates (35 μg/lane). This demonstrates the H2AFX antibody detected the H2AFX protein (arrow).
