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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- ARG55251 - Provider product page
- Provider
- Arigo
- Product name
- anti-Histone H2A.X phospho (Ser139) antibody
- Antibody type
- Monoclonal
- Antigen
- Synthetic phosphospecific peptide around Ser139 of Human H2A.X.
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A9-B5
- Vial size
- 100 µl
- Concentration
- 4.2 mg/ml
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 3T3 or Hydroxyurea-treated 3T3 cell lysates stained with ARG55251 anti-Histone H2A.X phospho (Ser139) antibody at 1:2000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: 3T3 or Hydroxyurea-treated 3T3 cells stained with ARG55251 anti-Histone H2A.X phospho (Ser139) antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: A549 (upper, untreated or Hydroxyurea-treated) and HeLa (lower, untreated or Hydroxyurea-treated) stained with ARG55251 anti-Histone H2A.X phospho (Ser139) antibody at 1:400 dilution.
