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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28785 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2A.X Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HCT116 control. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.66 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Histone H2A. X Polyclonal Antibody (Product # PA5-28785). Sample (30 µg of whole cell lysate). Lane A: HCT116. 15% SDS PAGE. Histone H2A. X Polyclonal Antibody (Product # PA5-28785) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A. X Polyclonal Antibody detects Histone H2A. X protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A. X protein stained by Histone H2A. X Polyclonal Antibody (Product # PA5-28785) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AGS xenograft, using Histone H2A. X (Product # PA5-28785) antibody at 1:1,000 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
