Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-383 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-383, RRID:AB_10002060
- Product name
- Rabbit Polyclonal Histone H2AX Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This is specific for H2AX. It is not known if this also recognizes the phosphorylated form of H2AX.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis.
Combined inhibition of cell death induced by apoptosis inducing factor and caspases provides additive neuroprotection in experimental traumatic brain injury.
Critical role of monoubiquitination of histone H2AX protein in histone H2AX phosphorylation and DNA damage response.
Combination of nutlin-3 and VX-680 selectively targets p53 mutant cells with reversible effects on cells expressing wild-type p53.
AIF promotes chromatinolysis and caspase-independent programmed necrosis by interacting with histone H2AX.
WRN is required for ATM activation and the S-phase checkpoint in response to interstrand cross-link-induced DNA double-strand breaks.
Examining the link between chromosomal instability and aneuploidy in human cells.
Developmental pluripotency-associated 4 (DPPA4) localized in active chromatin inhibits mouse embryonic stem cell differentiation into a primitive ectoderm lineage.
Cross-species difference in telomeric function of tankyrase 1.
Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeres.
The telomerase reverse transcriptase regulates chromatin state and DNA damage responses.
Leimbacher PA, Jones SE, Shorrocks AK, de Marco Zompit M, Day M, Blaauwendraad J, Bundschuh D, Bonham S, Fischer R, Fink D, Kessler BM, Oliver AW, Pearl LH, Blackford AN, Stucki M
Molecular cell 2019 May 2;74(3):571-583.e8
Molecular cell 2019 May 2;74(3):571-583.e8
Combined inhibition of cell death induced by apoptosis inducing factor and caspases provides additive neuroprotection in experimental traumatic brain injury.
Piao CS, Loane DJ, Stoica BA, Li S, Hanscom M, Cabatbat R, Blomgren K, Faden AI
Neurobiology of disease 2012 Jun;46(3):745-58
Neurobiology of disease 2012 Jun;46(3):745-58
Critical role of monoubiquitination of histone H2AX protein in histone H2AX phosphorylation and DNA damage response.
Wu CY, Kang HY, Yang WL, Wu J, Jeong YS, Wang J, Chan CH, Lee SW, Zhang X, Lamothe B, Campos AD, Darnay BG, Lin HK
The Journal of biological chemistry 2011 Sep 2;286(35):30806-15
The Journal of biological chemistry 2011 Sep 2;286(35):30806-15
Combination of nutlin-3 and VX-680 selectively targets p53 mutant cells with reversible effects on cells expressing wild-type p53.
Cheok CF, Kua N, Kaldis P, Lane DP
Cell death and differentiation 2010 Sep;17(9):1486-500
Cell death and differentiation 2010 Sep;17(9):1486-500
AIF promotes chromatinolysis and caspase-independent programmed necrosis by interacting with histone H2AX.
Artus C, Boujrad H, Bouharrour A, Brunelle MN, Hoos S, Yuste VJ, Lenormand P, Rousselle JC, Namane A, England P, Lorenzo HK, Susin SA
The EMBO journal 2010 May 5;29(9):1585-99
The EMBO journal 2010 May 5;29(9):1585-99
WRN is required for ATM activation and the S-phase checkpoint in response to interstrand cross-link-induced DNA double-strand breaks.
Cheng WH, Muftic D, Muftuoglu M, Dawut L, Morris C, Helleday T, Shiloh Y, Bohr VA
Molecular biology of the cell 2008 Sep;19(9):3923-33
Molecular biology of the cell 2008 Sep;19(9):3923-33
Examining the link between chromosomal instability and aneuploidy in human cells.
Thompson SL, Compton DA
The Journal of cell biology 2008 Feb 25;180(4):665-72
The Journal of cell biology 2008 Feb 25;180(4):665-72
Developmental pluripotency-associated 4 (DPPA4) localized in active chromatin inhibits mouse embryonic stem cell differentiation into a primitive ectoderm lineage.
Masaki H, Nishida T, Kitajima S, Asahina K, Teraoka H
The Journal of biological chemistry 2007 Nov 9;282(45):33034-42
The Journal of biological chemistry 2007 Nov 9;282(45):33034-42
Cross-species difference in telomeric function of tankyrase 1.
Muramatsu Y, Ohishi T, Sakamoto M, Tsuruo T, Seimiya H
Cancer science 2007 Jun;98(6):850-7
Cancer science 2007 Jun;98(6):850-7
Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeres.
Hovest MG, Brüggenolte N, Hosseini KS, Krieg T, Herrmann G
Molecular biology of the cell 2006 Apr;17(4):1758-67
Molecular biology of the cell 2006 Apr;17(4):1758-67
The telomerase reverse transcriptase regulates chromatin state and DNA damage responses.
Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC
Proceedings of the National Academy of Sciences of the United States of America 2005 Jun 7;102(23):8222-7
Proceedings of the National Academy of Sciences of the United States of America 2005 Jun 7;102(23):8222-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from 293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: H2AX Antibody [NB100-383] - Whole cell lysate (15 ug) from NIH3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Histone H2AX Antibody [NB100-383] - Simple Western lane view shows a specific band for H2AX in 0.2 mg/ml of HEK lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Histone H2AX Antibody [NB100-383] - Electropherogram image(s) of corresponding Simple Western lane view. H2AX antibody was used at 10 ug/ml dilution on HEK lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H2AX Antibody [NB100-383] - Detection of mouse H2AX by western blot. Samples: Whole cell lysate (50 ug) from F9 and L1210 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: H2AX Antibody [NB100-383] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX (NB100-383). Detection: DAB