Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA1-41004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2A.X Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: Jurkat or PBMC.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Skin models for cutaneous melioidosis reveal Burkholderia infection dynamics at wound's edge with inflammasome activation, keratinocyte extrusion and epidermal detachment.
82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.
Ku JWK, Marsh ST, Nai MH, Robinson KS, Teo DET, Zhong FL, Brown KA, Lim TC, Lim CT, Gan YH
Emerging microbes & infections 2021 Dec;10(1):2326-2339
Emerging microbes & infections 2021 Dec;10(1):2326-2339
82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.
Winick-Ng W, Caetano FA, Winick-Ng J, Morey TM, Heit B, Rylett RJ
Scientific reports 2016 Apr 7;6:23914
Scientific reports 2016 Apr 7;6:23914
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3) and COS-7 (Lane 4). The blot was probed with Anti-Histone H2A.X Polyclonal Antibody (Product # PA1-41004, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to H2A.X was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H2A.X in human PBMC lysate. Sample was incubated in Histone H2A.X polyclonal antibody (Product # PA1-41004).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3) and COS-7 (Lane 4). The blot was probed with Anti-Histone H2A.X Polyclonal Antibody (Product # PA1-41004, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to H2A.X was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Histone H2A.X in Human breast. Samples were incubated with Histone H2A.X polyclonal antibody (Product # PA1-41004) using a dilution of 5 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous Histone 1F0 protein at specific gene loci using Anti-Histone 1F0 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone 1F0 Polyclonal Antibody (Product # PA5-51466, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the GAPDH gene (active) and MYOD, SAT2 satellite repeats, SAT Alpha (inactive). A schematic diagram of the GAPDH gene is shown on top of the figure. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.