Antibody data
- Antibody Data
- Antigen structure
- References [30]
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- Validations
- Western blot [2]
- Other assay [17]
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- Product number
- PA1-988 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAPDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues..
- Concentration
- 1 mg/mL
Submitted references Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy.
CircRNA circYY1 (hsa_circ_0101187) Modulates Cell Glycolysis and Malignancy Through Regulating YY1 Expression by Sponging miR-769-3p in Breast Cancer.
Hepatic expression of GAA results in enhanced enzyme bioavailability in mice and non-human primates.
Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers.
Gene therapy with secreted acid alpha-glucosidase rescues Pompe disease in a novel mouse model with early-onset spinal cord and respiratory defects.
DACH1, a novel target of miR-218, participates in the regulation of cell viability, apoptosis, inflammatory response, and epithelial-mesenchymal transition process in renal tubule cells treated by high-glucose.
Why Does Knocking Out NACHO, But Not RIC3, Completely Block Expression of α7 Nicotinic Receptors in Mouse Brain?
SQSTM1/p62 and PPARGC1A/PGC-1alpha at the interface of autophagy and vascular senescence.
Small molecular compounds efficiently convert human fibroblasts directly into neurons.
BACE1 Inhibition Using 2'-OMePS Steric Blocking Antisense Oligonucleotides.
Engineered nanomedicine for neuroregeneration: light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factor.
Deep morphological analysis of muscle biopsies from type III glycogenesis (GSDIII), debranching enzyme deficiency, revealed stereotyped vacuolar myopathy and autophagy impairment.
AAV Gene Transfer with Tandem Promoter Design Prevents Anti-transgene Immunity and Provides Persistent Efficacy in Neonate Pompe Mice.
Role of spinal GABA receptors in the acute antinociceptive response of mice to hyperbaric oxygen.
Downregulation of ROS1 enhances the therapeutic efficacy of arsenic trioxide in acute myeloid leukemia cell lines.
A role for proteolytic regulation of δ-catenin in remodeling a subpopulation of dendritic spines in the rodent brain.
Yersinia pestis Targets the Host Endosome Recycling Pathway during the Biogenesis of the Yersinia-Containing Vacuole To Avoid Killing by Macrophages.
Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors.
Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice.
(-)-Epicatechin induces physiological cardiac growth by activation of the PI3K/Akt pathway in mice.
Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk.
Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor γ in MC3T3-E1 cells.
MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ-dependent manner.
Registered report: A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations.
Intermittent hypoxia selects for genotypes and phenotypes that increase survival, invasion, and therapy resistance.
Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells.
Selective in vivo targeting of human liver tumors by optimized AAV3 vectors in a murine xenograft model.
Calpains mediate axonal cytoskeleton disintegration during Wallerian degeneration.
Calpastatin overexpression protects axonal transport in an in vivo model of traumatic axonal injury.
The cannabinoid CB1 receptor antagonist rimonabant (SR141716) inhibits cell proliferation and increases markers of adipocyte maturation in cultured mouse 3T3 F442A preadipocytes.
Liu P, Li X, Liu J, Zhang H, You Z, Zhang J
BMC neuroscience 2022 Jul 1;23(1):42
BMC neuroscience 2022 Jul 1;23(1):42
CircRNA circYY1 (hsa_circ_0101187) Modulates Cell Glycolysis and Malignancy Through Regulating YY1 Expression by Sponging miR-769-3p in Breast Cancer.
Zhang X, Li J, Feng Q
Cancer management and research 2021;13:1145-1158
Cancer management and research 2021;13:1145-1158
Hepatic expression of GAA results in enhanced enzyme bioavailability in mice and non-human primates.
Costa-Verdera H, Collaud F, Riling CR, Sellier P, Nordin JML, Preston GM, Cagin U, Fabregue J, Barral S, Moya-Nilges M, Krijnse-Locker J, van Wittenberghe L, Daniele N, Gjata B, Cosette J, Abad C, Simon-Sola M, Charles S, Li M, Crosariol M, Antrilli T, Quinn WJ 3rd, Gross DA, Boyer O, Anguela XM, Armour SM, Colella P, Ronzitti G, Mingozzi F
Nature communications 2021 Nov 4;12(1):6393
Nature communications 2021 Nov 4;12(1):6393
Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers.
Li Y, Gong H, Feng L, Mao D, Xiao Y, Wang Y, Huang L
FEBS open bio 2021 Feb;11(2):423-434
FEBS open bio 2021 Feb;11(2):423-434
Gene therapy with secreted acid alpha-glucosidase rescues Pompe disease in a novel mouse model with early-onset spinal cord and respiratory defects.
Colella P, Sellier P, Gomez MJ, Biferi MG, Tanniou G, Guerchet N, Cohen-Tannoudji M, Moya-Nilges M, van Wittenberghe L, Daniele N, Gjata B, Krijnse-Locker J, Collaud F, Simon-Sola M, Charles S, Cagin U, Mingozzi F
EBioMedicine 2020 Nov;61:103052
EBioMedicine 2020 Nov;61:103052
DACH1, a novel target of miR-218, participates in the regulation of cell viability, apoptosis, inflammatory response, and epithelial-mesenchymal transition process in renal tubule cells treated by high-glucose.
Zhang YL, Wang JM, Yin H, Wang SB, He CL, Liu J
Renal failure 2020 Nov;42(1):463-473
Renal failure 2020 Nov;42(1):463-473
Why Does Knocking Out NACHO, But Not RIC3, Completely Block Expression of α7 Nicotinic Receptors in Mouse Brain?
Deshpande A, Vinayakamoorthy RM, Garg BK, Thummapudi JP, Oza G, Adhikari K, Agarwal A, Dalvi P, Iyer S, Thulasi Raman S, Ramesh V, Rameshbabu A, Rezvaya A, Sukumaran S, Swaminathan S, Tilak B, Wang Z, Tran PV, Loring RH
Biomolecules 2020 Mar 19;10(3)
Biomolecules 2020 Mar 19;10(3)
SQSTM1/p62 and PPARGC1A/PGC-1alpha at the interface of autophagy and vascular senescence.
Salazar G, Cullen A, Huang J, Zhao Y, Serino A, Hilenski L, Patrushev N, Forouzandeh F, Hwang HS
Autophagy 2020 Jun;16(6):1092-1110
Autophagy 2020 Jun;16(6):1092-1110
Small molecular compounds efficiently convert human fibroblasts directly into neurons.
Yang J, Cao H, Guo S, Zhu H, Tao H, Zhang L, Chen Z, Sun T, Chi S, Hu Q
Molecular medicine reports 2020 Dec;22(6):4763-4771
Molecular medicine reports 2020 Dec;22(6):4763-4771
BACE1 Inhibition Using 2'-OMePS Steric Blocking Antisense Oligonucleotides.
Chakravarthy M, Veedu RN
Genes 2019 Sep 12;10(9)
Genes 2019 Sep 12;10(9)
Engineered nanomedicine for neuroregeneration: light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factor.
Yuan M, Wang Y, Qin YX
Nanomedicine : nanotechnology, biology, and medicine 2019 Oct;21:102052
Nanomedicine : nanotechnology, biology, and medicine 2019 Oct;21:102052
Deep morphological analysis of muscle biopsies from type III glycogenesis (GSDIII), debranching enzyme deficiency, revealed stereotyped vacuolar myopathy and autophagy impairment.
Laforêt P, Inoue M, Goillot E, Lefeuvre C, Cagin U, Streichenberger N, Leonard-Louis S, Brochier G, Madelaine A, Labasse C, Hedberg-Oldfors C, Krag T, Jauze L, Fabregue J, Labrune P, Milisenda J, Nadaj-Pakleza A, Sacconi S, Mingozzi F, Ronzitti G, Petit F, Schoser B, Oldfors A, Vissing J, Romero NB, Nishino I, Malfatti E
Acta neuropathologica communications 2019 Oct 28;7(1):167
Acta neuropathologica communications 2019 Oct 28;7(1):167
AAV Gene Transfer with Tandem Promoter Design Prevents Anti-transgene Immunity and Provides Persistent Efficacy in Neonate Pompe Mice.
Colella P, Sellier P, Costa Verdera H, Puzzo F, van Wittenberghe L, Guerchet N, Daniele N, Gjata B, Marmier S, Charles S, Simon Sola M, Ragone I, Leborgne C, Collaud F, Mingozzi F
Molecular therapy. Methods & clinical development 2019 Mar 15;12:85-101
Molecular therapy. Methods & clinical development 2019 Mar 15;12:85-101
Role of spinal GABA receptors in the acute antinociceptive response of mice to hyperbaric oxygen.
Brewer AL, Liu S, Buhler AV, Shirachi DY, Quock RM
Brain research 2018 Nov 15;1699:107-116
Brain research 2018 Nov 15;1699:107-116
Downregulation of ROS1 enhances the therapeutic efficacy of arsenic trioxide in acute myeloid leukemia cell lines.
Li J
Oncology letters 2018 Jun;15(6):9392-9396
Oncology letters 2018 Jun;15(6):9392-9396
A role for proteolytic regulation of δ-catenin in remodeling a subpopulation of dendritic spines in the rodent brain.
Yuan L, Singh D, Buescher JL, Arikkath J
The Journal of biological chemistry 2018 Jul 20;293(29):11625-11638
The Journal of biological chemistry 2018 Jul 20;293(29):11625-11638
Yersinia pestis Targets the Host Endosome Recycling Pathway during the Biogenesis of the Yersinia-Containing Vacuole To Avoid Killing by Macrophages.
Connor MG, Pulsifer AR, Chung D, Rouchka EC, Ceresa BK, Lawrenz MB
mBio 2018 Feb 20;9(1)
mBio 2018 Feb 20;9(1)
Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors.
Garg BK, Loring RH
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Sep;65(9):499-512
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Sep;65(9):499-512
Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice.
Longo F, Mercatelli D, Novello S, Arcuri L, Brugnoli A, Vincenzi F, Russo I, Berti G, Mabrouk OS, Kennedy RT, Shimshek DR, Varani K, Bubacco L, Greggio E, Morari M
Acta neuropathologica communications 2017 Mar 14;5(1):22
Acta neuropathologica communications 2017 Mar 14;5(1):22
(-)-Epicatechin induces physiological cardiac growth by activation of the PI3K/Akt pathway in mice.
De Los Santos S, García-Pérez V, Hernández-Reséndiz S, Palma-Flores C, González-Gutiérrez CJ, Zazueta C, Canto P, Coral-Vázquez RM
Molecular nutrition & food research 2017 Feb;61(2)
Molecular nutrition & food research 2017 Feb;61(2)
Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk.
Koch F, Lamp O, Eslamizad M, Weitzel J, Kuhla B
PloS one 2016;11(8):e0160912
PloS one 2016;11(8):e0160912
Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor γ in MC3T3-E1 cells.
Qu B, Ma Y, Yan M, Gong K, Liang F, Deng S, Jiang K, Ma Z, Pan X
Biochemical and biophysical research communications 2016 Sep 9;478(1):439-445
Biochemical and biophysical research communications 2016 Sep 9;478(1):439-445
MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ-dependent manner.
Gong K, Qu B, Liao D, Liu D, Wang C, Zhou J, Pan X
Biochemical and biophysical research communications 2016 Sep 9;478(1):260-267
Biochemical and biophysical research communications 2016 Sep 9;478(1):260-267
Registered report: A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations.
Haven B, Heilig E, Donham C, Settles M, Vasilevsky N, Owen K, Reproducibility Project: Cancer Biology, Reproducibility Project Cancer Biology
eLife 2016 Feb 23;5
eLife 2016 Feb 23;5
Intermittent hypoxia selects for genotypes and phenotypes that increase survival, invasion, and therapy resistance.
Verduzco D, Lloyd M, Xu L, Ibrahim-Hashim A, Balagurunathan Y, Gatenby RA, Gillies RJ
PloS one 2015;10(3):e0120958
PloS one 2015;10(3):e0120958
Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells.
Goutzourelas N, Stagos D, Housmekeridou A, Karapouliou C, Kerasioti E, Aligiannis N, Skaltsounis AL, Spandidos DA, Tsatsakis AM, Kouretas D
International journal of molecular medicine 2015 Aug;36(2):433-41
International journal of molecular medicine 2015 Aug;36(2):433-41
Selective in vivo targeting of human liver tumors by optimized AAV3 vectors in a murine xenograft model.
Ling C, Wang Y, Zhang Y, Ejjigani A, Yin Z, Lu Y, Wang L, Wang M, Li J, Hu Z, Aslanidi GV, Zhong L, Gao G, Srivastava A, Ling C
Human gene therapy 2014 Dec;25(12):1023-34
Human gene therapy 2014 Dec;25(12):1023-34
Calpains mediate axonal cytoskeleton disintegration during Wallerian degeneration.
Ma M, Ferguson TA, Schoch KM, Li J, Qian Y, Shofer FS, Saatman KE, Neumar RW
Neurobiology of disease 2013 Aug;56:34-46
Neurobiology of disease 2013 Aug;56:34-46
Calpastatin overexpression protects axonal transport in an in vivo model of traumatic axonal injury.
Ma M, Shofer FS, Neumar RW
Journal of neurotrauma 2012 Nov 1;29(16):2555-63
Journal of neurotrauma 2012 Nov 1;29(16):2555-63
The cannabinoid CB1 receptor antagonist rimonabant (SR141716) inhibits cell proliferation and increases markers of adipocyte maturation in cultured mouse 3T3 F442A preadipocytes.
Gary-Bobo M, Elachouri G, Scatton B, Le Fur G, Oury-Donat F, Bensaid M
Molecular pharmacology 2006 Feb;69(2):471-8
Molecular pharmacology 2006 Feb;69(2):471-8
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- Western blot analysis of GAPDH was performed by loading 25 µg of Hela (lane 1) and C6 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a GAPDH polyclonal antibody (Product # PA1-988) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~36 kDa.
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- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), HT-29 (Lane 4), C2C12 (Lane 5), PC-12 (Lane 6), U-2 OS (Lane 7) and tissue extract (30 µg lysate) of Rat Testis (Lane 8), Mouse Liver (Lane 9). The blot was probed with Anti-GAPDH Polyclonal Antibody (Product # PA1-988, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to GAPDH was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
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- Fig 4 Differential protein levels and phenotypes are exhibited among MCF10A cells following selection through IH. (A) Analysis of protein levels was performed using the Reverse Phase Protein Array (RPPA) on the parental MCF10A line, passaged control clone 7, IH-selected clone 4, and IH-selected clone 9. Each clone was submitted in duplicate. Hierarchical clustering of significant (FDR
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- Figure 1 Representative western blots showing the effects of grape pomace extract (GPE) on the expression of (A) gamma-glutamylcysteine synthetase (GCS), (B) catalase (CAT), (C) superoxide dismutase (SOD) and (D) heme oxygenase 1 (HO-1) in C2C12 muscle cells. The results of densitometric quantification for all enzymes are also shown. The cells were incubated with GPE at 2.5 and 10 u g/ml for 3, 6, 12, 18 and 24 h. The expression of GAPDH was used as a loading control for normalization. * P
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- Figure 2 Representative western blots showing the effects of grape pomace extract (GPE) on the expression of (A) gamma-glutamylcysteine synthetase (GCS), (B) catalase (CAT), (C) superoxide dismutase (SOD) and (D) heme oxygenase 1 (HO-1) in EA.hy926 endothelial cells. The results of densitometric quantification for all enzymes are also shown. The cells were incubated with GPE at 0.068 and 0.250 u g/ml for 3, 6, 12, 18 and 24 h. The expression of GAPDH was used as a loading control for normalization. * P
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- Fig. 4 Gene therapy with AAV vectors encoding for a secretable GAA variant rapidly rescues skeletal muscle defects in Gaa KO DBA mice. Fig. 4 (a) Study diagram: Gaa KO DBA mice were treated by intravenous injection of AAV9 vectors encoding for a codon-optimized secretable human GAA (secGAA) under the control of the tandem liver-muscle LiMP promoter (AAV-secGAA, dose: 4 x 10 10 vg/mouse, ~2 x 10 13 vg/kg); littermate untreated (no Tx) Gaa KO DBA and Gaa WT DBA were used as controls. Vectors were delivered at postnatal day 2 (P2), analyses were performed 4 months (4M) later; SkM (skeletal muscle), SC (spinal cord). Biochemical (b-g) and downstream analysis of RNA sequencing (RNA-seq) data (h-i) of skeletal muscle (SkM) at the end of the study. (b) Analyses of GAA enzyme activity in triceps muscle. (c) Analyses of glycogen storage in triceps muscle. (d) Western blot analyses of triceps lysates with anti-human GAA, anti-Parkin, and anti-p62 antibodies, anti-Gapdh antibody was used as loading control. The molecular weight marker (kDa) is depicted. The lanes were run on the same gel but were non-contiguous. Western blot quantification is shown in panels e (GAA), f (p62) and g (Parkin). (b-g) Gaa WT DBA ( n = 4), Gaa KO DBA ( n = 5), Gaa KO DBA +AAV ( n = 4). Data are depicted as average+-SD. (h) Gene expression clusters. Expression profiles for 2573 DEGs with abs (logFC) > 0.5, detected in contrast untreated Gaa KO DBA vs. Gaa WT DBA , were constructed by recovering averaged expres
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- Figure 3. Characteristics of iNs. (A) iNs exhibited bipolar neuronal morphologies and expressed tuj1 (red) and MAP2 (green) at different phases (day 7, 14 and 30). (B) iNs were stained for NeuN, GABA, vGLUT1 and GFAP (all green) on day 30. Scale bar, 50 um. (C) Reverse transcription-quantitative PCR analysis revealed that in comparison with HFFs, there was a significant increase in gene expression levels of MAP2, DCX, NeuroD1 and ASCL1 in iNs, whereas human fibroblast-specific genes were downregulated in iNs on day 30. Data are presented as the mean +- SD. (D) Western blot analysis of the protein expression levels of Tuj1, NeuN and GAPDH in HFFs and iNs. (E) Graphs show the semi-quantification of the western blot analysis. n=3 independent experiments. Data are presented as the mean +- SD. Unpaired Student's t-tests were used to compare data. *P
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- Fig. 9 SPK-3006 drives vector dose-dependent expression of GAA in liver and tissue uptake in non-human primates. A Circulating GAA antigen levels 4 weeks of post-injection of C57BL/6 mice with AAV-GAA or SPK-3006 at 2 x 10 12 vg/kg ( n = 5 per group). B GAA activity in plasma of non-human primates treated with SPK-3006 ( n = 4 per group). C , E Western blot analysis of GAA antigen levels in spinal cord ( C ) and brain ( E ). D , F Quantification of lysosomal GAA antigen in spinal cord ( D ) and brain ( F ) (2 x 10 12 and 6 x 10 12 vg/kg, n = 4 per group; 2 x 10 13 , n = 3). Data shown as average +- SD. Statistical analysis: A Two-sided Mann-Whitney t -test; B Two-way ANOVA with Tukey post-hoc test; D , F One-way ANOVA.
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- Fig. 1 Phosphorylation levels of LRRK2 at Ser1292 (pSer1292) are elevated in G2019S knock-in (KI) mice. Striatal pSer1292 and total LRRK2 levels were measured by Western blotting in 12-month-old G2019S KI mice and age-matched WT controls. Representative blots (left) and quantification (right) are shown. Data are expressed as pSer1292 LRRK2/total LRRK2 and are means +- SEM of 7 animals per group. Statistical analysis was performed with the Student t -test, two tailed for unpaired data. ** p < 0.01, different from WT
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- Fig. 8 Autophagic flux studies in GSDIII mouse tissues. a. Augmented glycogen content in both GDE KO mice of 14 and 20 months . b. SQSTM1/p62 protein by immunostaining of total protein lysates of quadriceps tissues showing variable levels of SQSTM1/p62 proteinold mice. c. p62/SQSTM1 protein by immunostaining of total protein lysates of quadriceps tissues in 20 months GSDIII mice showing slightly decreased level of p62
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- Figure 1 ( A ). Western Blot analysis of NACHO expression. GH3 and GH4C1 cells express endogenous NACHO, but mouse macrophage-like RAW264.7 cells, primary mouse macrophages, and HEK-293 do not. ( B ) In vitro 125 I-alphaBGT binding assessments. Primary cultured macrophages and GH3 cells transfected with rat chrna7 plasmid, but not RAW264.7 mouse macrophage-derived cells, showed 125 I-alphaBGT binding.