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LearnBS4238
antibody from Bioworld Technology, Inc
Targeting: IKBKG
FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- BS4238 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- IKKÎÛ (phospho S31) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human IKKÎÛ around the phosphorylation site of Serine 31.
- Description
- Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase Ï« (IKKÏ«), previously designated CHUK, interacts with IkB-Ï« and specifically phosphorylates I?BÏ« on Serine 32 and 36, the sites that trigger its degradation. IKKÏ« appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKÏ« is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKÏ«, IKKÉÇ and IKKÎÛ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of p-IKK¦Ã (S31) pAb at 1:500 dilutionLane1:HEK293T cell lysate treated with TNFa(20ng/ml,15mins)Lane2:Raw264.7 cell lysate treated with TNFa(20ng/ml,15mins)Lane3:PC12 cell lysate treated with TNFa(20ng/ml,15mins)
