MA11C5

antibody from Invitrogen Antibodies

Targeting: ITGAX CD11C

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA11C5

Provider

Invitrogen Antibodies

Product name

Anti-CD11c Monoclonal Antibody (N418)

Antibody type

Monoclonal

Reactivity

Human, Mouse

Isotype

IgG

Antibody clone number

N418

Vial size

500 ug

Concentration

1.03 mg/ml

Storage

-20°C

References

Submitted references

Inhibition of Signal Transducer and Activator of Transcription 3 (STAT3) reduces neonatal hypoxic-ischaemic brain damage.

Hristova M, Rocha-Ferreira E, Fontana X, Thei L, Buckle R, Christou M, Hompoonsup S, Gostelow N, Raivich G, Peebles D
Journal of neurochemistry 2016 Mar;136(5):981-94

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The pathogenesis of severe fever with thrombocytopenia syndrome virus infection in alpha/beta interferon knockout mice: insights into the pathologic mechanisms of a new viral hemorrhagic fever.

Liu Y, Wu B, Paessler S, Walker DH, Tesh RB, Yu XJ
Journal of virology 2014 Feb;88(3):1781-6

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Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis.

Reeh KA, Cardenas KT, Bain VE, Liu Z, Laurent M, Manley NR, Richie ER
Development (Cambridge, England) 2014 Aug;141(15):2950-8

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Maternal immune stimulation during pregnancy shapes the immunological phenotype of offspring.

Mandal M, Donnelly R, Elkabes S, Zhang P, Davini D, David BT, Ponzio NM
Brain, behavior, and immunity 2013 Oct;33:33-45

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TIM-3 regulates innate immune cells to induce fetomaternal tolerance.

Chabtini L, Mfarrej B, Mounayar M, Zhu B, Batal I, Dakle PJ, Smith BD, Boenisch O, Najafian N, Akiba H, Yagita H, Guleria I
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):88-96

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The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain.

Kanu N, Penicud K, Hristova M, Wong B, Irvine E, Plattner F, Raivich G, Behrens A
The Journal of biological chemistry 2010 Dec 3;285(49):38534-42

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Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver.

Lloyd CM, Phillips AR, Cooper GJ, Dunbar PR
Journal of immunological methods 2008 May 20;334(1-2):70-81

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Cigarette smoke exposure impairs dendritic cell maturation and T cell proliferation in thoracic lymph nodes of mice.

Robbins CS, Franco F, Mouded M, Cernadas M, Shapiro SD
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6623-8

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CD11c/EYFP transgene illuminates a discrete network of dendritic cells within the embryonic, neonatal, adult, and injured mouse brain.

Bulloch K, Miller MM, Gal-Toth J, Milner TA, Gottfried-Blackmore A, Waters EM, Kaunzner UW, Liu K, Lindquist R, Nussenzweig MC, Steinman RM, McEwen BS
The Journal of comparative neurology 2008 Jun 10;508(5):687-710

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Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.

Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, Höpner S, Centonze D, Bernardi G, Dell'Acqua ML, Rossini PM, Battistini L, Rötzschke O, Falk K
Blood 2007 Aug 15;110(4):1225-32

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Type I interferon dependence of plasmacytoid dendritic cell activation and migration.

Asselin-Paturel C, Brizard G, Chemin K, Boonstra A, O'Garra A, Vicari A, Trinchieri G
The Journal of experimental medicine 2005 Apr 4;201(7):1157-67

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Study on pancreatic lymphatics in nonobese diabetic mouse with prevention of insulitis and diabetes by adjuvant immunotherapy.

Qu P, Ji RC, Shimoda H, Miura M, Kato S
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 2004 Dec;281(2):1326-36

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Identification of a novel macrophage population in the normal mouse corneal stroma.

Brissette-Storkus CS, Reynolds SM, Lepisto AJ, Hendricks RL
Investigative ophthalmology & visual science 2002 Jul;43(7):2264-71

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Immunization with the adjuvant MF59 induces macrophage trafficking and apoptosis.

Dupuis M, Denis-Mize K, LaBarbara A, Peters W, Charo IF, McDonald DM, Ott G
European journal of immunology 2001 Oct;31(10):2910-8

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Tissue distribution of products of the mouse decay-accelerating factor (DAF) genes. Exploitation of a Daf1 knock-out mouse and site-specific monoclonal antibodies.

Lin F, Fukuoka Y, Spicer A, Ohta R, Okada N, Harris CL, Emancipator SN, Medof ME
Immunology 2001 Oct;104(2):215-25

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Brain dendritic cells and macrophages/microglia in central nervous system inflammation.

Fischer HG, Reichmann G
Journal of immunology (Baltimore, Md. : 1950) 2001 Feb 15;166(4):2717-26

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Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with Toxoplasma gondii.

Fischer HG, Bonifas U, Reichmann G
Journal of immunology (Baltimore, Md. : 1950) 2000 May 1;164(9):4826-34

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Generation of tumor immunity by bone marrow-derived dendritic cells correlates with dendritic cell maturation stage.

Labeur MS, Roters B, Pers B, Mehling A, Luger TA, Schwarz T, Grabbe S
Journal of immunology (Baltimore, Md. : 1950) 1999 Jan 1;162(1):168-75

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Antigen presentation function of brain-derived dendriform cells depends on astrocyte help.

Fischer HG, Bielinsky AK
International immunology 1999 Aug;11(8):1265-74

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Ultraviolet light-induced immune tolerance is mediated via the Fas/Fas-ligand system.

Schwarz A, Grabbe S, Grosse-Heitmeyer K, Roters B, Riemann H, Luger TA, Trinchieri G, Schwarz T
Journal of immunology (Baltimore, Md. : 1950) 1998 May 1;160(9):4262-70

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Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.

Liu L, Rich BE, Inobe J, Chen W, Weiner HL
International immunology 1998 Aug;10(8):1017-26

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CD14 is expressed by subsets of murine dendritic cells and upregulated by lipopolysaccharide.

Mahnke K, Becher E, Ricciardi-Castagnoli P, Luger TA, Schwarz T, Grabbe S
Advances in experimental medicine and biology 1997;417:145-59

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The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.

Inaba K, Witmer-Pack M, Inaba M, Hathcock KS, Sakuta H, Azuma M, Yagita H, Okumura K, Linsley PS, Ikehara S, Muramatsu S, Hodes RJ, Steinman RM
The Journal of experimental medicine 1994 Nov 1;180(5):1849-60

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The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.

Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM
The Journal of experimental medicine 1990 May 1;171(5):1753-71

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD11c (green) showing staining in the cytoplasm and membrane of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD11c monoclonal antibody (Product # MA11C5) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD11c (green) showing staining in the membrane of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD11c monoclonal antibody (Product # MA11C5) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD11c in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA11C5) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4¼C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4¼C in the dark. FACS analysis was performed using 400 ul of cell buffer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD11c in HL-60 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD11c monoclonal antibody (Product # MA11C5) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.