MA3-928

antibody from Invitrogen Antibodies

Targeting: ATP1A1

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA3-928 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATP1A1 Monoclonal Antibody (M7-PB-E9)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA3-928 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only. MA3-928 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, immunocytochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha sodium/potassium ATPase from MDCK cell extract. Immunohistochemical staining of alpha sodium/potassium ATPase in porcine heart with MA3-928 yields a pattern consistent with the plasma membrane localization. The MA3-928 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine
Host: Mouse
Isotype: IgG
Antibody clone number: M7-PB-E9
Vial size: 400 µL
Concentration: 1.87 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

ATP1A1 protein structure - MA3-928 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sodiµm/Potassium ATPase alpha using Sodiµm/Potassium ATPase alpha Monoclonal Antibody (M7-PB-E9) (Product # MA3-928) shows staining in Hela Cells. Sodiµm/Potassium ATPase alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Sodiµm/Potassium ATPase alpha (Product # MA3-928) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sodiµm/Potassium ATPase alpha using Sodiµm/Potassium ATPase alpha Monoclonal Antibody (M7-PB-E9) (Product # MA3-928) shows staining in MCF-7 Cells. Sodiµm/Potassium ATPase alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Sodiµm/Potassium ATPase alpha (Product # MA3-928) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sodiµm/Potassium ATPase alpha using Sodiµm/Potassium ATPase alpha Monoclonal Antibody (M7-PB-E9) (Product # MA3-928) shows staining in U251 Cells. Sodiµm/Potassium ATPase alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Sodiµm/Potassium ATPase alpha (Product # MA3-928) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 3 (A) Polarized CaCo-2w cells with localization of flotillin-1 (red), Na-K-ATPase (green) and flotillin-1 and Na-K-ATPase immunofluorescence overlay (right). Flotillin-1 and Na-K-ATPase co-localize in the basolateral membrane. (B) Confocal microscopy image of polarized CaCo-2w cells. Flotillin-1 (red) is localized in a subapical compartment, but not in the apical membrane. Na-K- ATPase (green) marks the basolateral membrane.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 6 Three-dimensional, confocal-microscopic images of a human colonic epithelial layer in a human endoscopic biopsy. Flotillin-1 is marked in red, Na-K-ATPase in green, immunofluorescence overlay on the right. Na-K-ATPase was used a marker of the basolateral cell membrane.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 8 Representative western blot image for detection of membrane associated TK1 levels in plasma membrane protein extracts from the non-small cell lung carcinoma cell line NCI-H460 and healthy mono nuclear cells. a Plasma membrane protein extract from NCI-H460 had high levels of membrane associate TK1. b Membrane associated TK1 was not detected in plasma membrane proteins extracts from healthy mono nuclear cells