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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA1-10046 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Actin Muscle Monoclonal Antibody (HHF35)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells. Western Blot: Reducing conditions; Immunohistochemistry (Paraffin): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Feline, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HHF35
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Rat Colon (Lane 6), Rat Pancreas (Lane 7), Mouse Kidney (Lane 8) and whole cell extract (30 µg lysate) of C2C12 (Lane 5). The blot was probed with Anti-Actin Muscle Monoclonal Antibody (HHF35) (Product # MA1-10046, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to beta Actin was observed in muscle specific tissues and cell line when compared to non muscle tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35 (Product # MA1-10046). Lane 1: murine femoral muscle. Lane 2: murine heart. Lane 3: HeLa. Lane 4: HUVEC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human smooth muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35) Monoclonal antibody (Product # MA1-10046).
