LS-C55561

antibody from LSBio

Targeting: MAP3K7 MEKK7, TAK1

ELISA

Antibody data

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Validation data

Product number: LS-C55561 - Provider product page
Provider: LSBio
Product name: MAP3K7 / TAK1 Antibody (aa525-537) LS-C55561
Antibody type: Polyclonal
Description: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Horse, Porcine, Rabbit, Simian, Xenopus
Host: Goat
Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

MAP3K7 protein structure - LS-C55561 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Antibody (1 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Antibody (1 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MAP3K7 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Goat Anti-TAK1 / MAP3K7 Antibody (1µg/ml) staining of HeLa (A) and U937 (B) and (2ug/ml) Daudi (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MAP3K7 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Goat Anti-TAK1 / MAP3K7 Antibody (1µg/ml) staining of HeLa (A) and U937 (B) and (2ug/ml) Daudi (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Antibody (1 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.