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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP12614PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12614PU-N, RRID:AB_1749987
- Product name
- anti CD309 / VEGFR-2 / Flk-1 pTyr996
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y996 of human KDR/FLK1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Anti-Phospho-KDR/FLK1-Y996 Pab (Cat.#AP12614PU-N) is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody (Cat#AP12614PU-N)diluted to 1:500 in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
