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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA501204 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501204, RRID:AB_11126591
- Product name
- DAPK2 (DAP Kinase 2 ) mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)
- Antibody type
- Monoclonal
- Description
- DAPK2 (DAP Kinase 2 ) mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DAPK2
- Isotype
- IgG
- Antibody clone number
- OTI1B10
- Vial size
- 100 µl
- Concentration
- 0.84 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAPK2 (RC216274, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-DAPK2 mouse monoclonal antibody (TA501204) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2(RC216274).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of DAPK2 by using TrueMab monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC216274 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DAPK2 antibody(TA501204), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-DAPK2 antibody(TA501204),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
