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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-21458 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A431, H1299, U87-MG, SK-N-SH, IMR32, SK-N-AS. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.37 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NEK4 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 15 min and stained using a NEK4 polyclonal antibody (Product # PA5-21458) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NEK4 Polyclonal Antibody detects NEK4 protein at nucleus by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 15 min. Green: NEK4 protein stained by NEK4 Polyclonal Antibody (Product # PA5-21458) diluted at 1:500. Blue: Hoechst 33343 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope].
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NEK4 Polyclonal Antibody detects NEK4 protein at nucleus by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 15 min. Green: NEK4 protein stained by NEK4 Polyclonal Antibody (Product # PA5-21458) diluted at 1:500. Blue: Hoechst 33343 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope].
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4 (Product # PA5-21458) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
