PA5-21458

antibody from Invitrogen Antibodies

Targeting: NEK4 NRK2, pp12301, STK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21458

Provider

Invitrogen Antibodies

Product name

NEK4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, U87-MG, SK-N-SH, IMR32, SK-N-AS.

Concentration

0.37 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NEK4 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NEK4 Polyclonal Antibody (Product # PA5-21458) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NEK4 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NEK4 Polyclonal Antibody (Product # PA5-21458) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NEK4 Polyclonal Antibody (Product # PA5-21458). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299 . 7.5% SDS PAGE. NEK4 Polyclonal Antibody (Product # PA5-21458) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NEK4 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 15 min and stained using a NEK4 polyclonal antibody (Product # PA5-21458) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NEK4 Polyclonal Antibody detects NEK4 protein at nucleus by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 15 min. Green: NEK4 protein stained by NEK4 Polyclonal Antibody (Product # PA5-21458) diluted at 1:500. Blue: Hoechst 33343 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope].

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4 (Product # PA5-21458) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.