NBP1-85784

antibody from Novus Biologicals

Targeting: IDH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85784

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85784, RRID:AB_11010140

Product name

Rabbit Polyclonal IDH2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human IDH2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Single muscle fiber proteomics reveals unexpected mitochondrial specialization.

Murgia M, Nagaraj N, Deshmukh AS, Zeiler M, Cancellara P, Moretti I, Reggiani C, Schiaffino S, Mann M
EMBO reports 2015 Mar;16(3):387-95

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-IDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human heart muscle shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human testis shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human colorectal cancer shows strong granular cytoplasmic positivity in tumor cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human prostate shows moderate to granular cytoplasmic positivity in smooth muscle cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human testis shows weak to moderate granular cytoplasmic positivity in a small subset of cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining in human heart muscle and testis tissues using NBP1-85784 antibody. Corresponding IDH2 RNA-seq data are presented for the same tissues.