MA5-14920

antibody from Invitrogen Antibodies

Targeting: STAT6 D12S1644, IL-4-STAT

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14920

Provider

Invitrogen Antibodies

Product name

Phospho-STAT6 (Tyr641) Monoclonal Antibody (J.187.3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with the corresponding phospho-tyrosine residues of other Stat proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

J.187.3

Vial size

100 μL

Concentration

103 μg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of phospho-STAT6 was performed using 70 confluent log phase ACHN cells upon treatment with IL-4. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-STAT6 (Tyr641) Monoclonal Antibody (J.187.3) (Product # MA5-14920, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents untreated ACHN cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.